Towards CRISPR-based editing of the mitochondrial genome in yeast.
Yin S, Jarosz DF, Ting AY
Proc Natl Acad Sci U S A. 2026 Jan 20;123(3):e2505894123. doi: 10.1073/pnas.2505894123. Epub 2026 Jan 16.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251972 | NLS-ABE8e-GFP | Yeast nDNA editing with ABE8e |
| 251973 | pADH1-MTS-ABE8e-P2A-EGFP | Yeast mtDNA editing with a mid-strength promoter for lower expression level |
| 251976 | MTS-ABE-ARG8 | Expression of ABE-ARG8 fusion protein in yeast |
| 251977 | nArg8-EGFP | Expression of nuclear ARG8-EGFP as control |
| 251978 | pPT24-mtArg8m*-V5 | For biolistic transformation to express Arg8m* from mtDNA |
| 251979 | pPT24-mtArg8m-V5 | For biolistic transformation to express Arg8m from mtDNA |
| 251982 | pPT24-mtArg8m*-V5-UTR-HH-ntsgRNA-HDV | For biolistic transformation to express Arg8m* and a non-targeting control sgRNA from mtDNA |
| 251984 | pRS426-SNR52-crRNA | Expression of crRNA (version 3) for editing, used together with tracrRNA Plasmid #251985. This plasmid is also called crRNA in the paper. |