Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1.
Falk JE, Henke T, Gowrisankaran S, Wanderoy S, Basu H, Greally S, Steen J, Schwarz TL
J Cell Biol. 2026 Apr 6;225(4):e202501023. doi: 10.1083/jcb.202501023. Epub 2026 Jan 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173069 | hSYN-mito-dsRED2 | mitochondria targeted dsRED2 expressed off of human Synapsin I promoter. |
| 173071 | CAG-mito-dsRED2 | mitochondria targeted dsRED2 expressed off of CAG promoter |
| 174541 | EF1a-mito-dsRED2 | mitochondria targeted dsRED expressed off of EF1a promoter |
| 176266 | iRFP-TRIM46 | TRIM46 tagged with iRFP702 |
| 188664 | meGFP-hTRAK1 | human TRAK1 with N-terminal meGFP tag |
| 219568 | pLVX-EF1a-mito-mRaspberry | Lentiviral construct expressing a mitochondria-targeted mRaspberry |
| 219569 | pLVX-CAG-Ftractin-GFP | Lentiviral construct expressing GFP-tagged F-tractin |
| 219570 | MYC-hTRAK1(S919A) | Expresses MYC-tagged hTRAK1 (S919A) |
| 219571 | MYC-hTRAK1(S719A) | Expresses MYC-tagged hTRAK1 (S719A) |
| 219572 | MYC-hTRAK1(S200A, S201A) | Expresses MYC-tagged hTRAK1 (S200A, S201A) |
| 219573 | MYC-hTRAK1(T834A) | Expresses MYC-tagged hTRAK1 (T834A) |
| 219574 | MYC-hTRAK1(S200A, S201A, S719A, T834A, S919A) | Expresses MYC-tagged hTRAK1 (S200A, S201A, S719A, T834A, S919A) |
| 219575 | pLVX-CAG-meGFP-Myo6 | Lentiviral construct expressing GFP tagged human Myo6 |
| 219576 | pLVX-CAG-Fhl2-HA-P2A-mitomeGFP | Lentiviral construct expressing HA-tagged Fhl2 followed by a P2A ribosome skip sequence and then a mitochondria targeted mEGFP. |
| 219577 | pLVX-hSYN-MYC-MIRO1 | Lentiviral construct expressing MYC-tagged human Miro1 |
| 225201 | pLVX-hSYN-meGFP | Lentiviral construct expressing meGFP from human synapsin promoter. |
| 225209 | pLVX-CAG-MCS | Lentiviral vector with CAG promoter and multiple cloning site |
| 225225 | hTRAK1(S200A, S201A, S719A, S919A) | Expresses MYC-tagged hTRAK1 (S200A, S201A, S719A, S919A) |
| 225226 | hTRAK1(S719A, S919A) | Expresses MYC-tagged hTRAK1(S719A, S919A) |
| 225227 | pCMV-meGFP-Myo6 | Expresses GFP-tagged Myo6 |
| 225231 | Fhl2-HA-P2A-mitomeGFP | Construct expressing HA-tagged Fhl2 followed by a P2A ribosome skip sequence and then a mitochondria targeted mEGFP |
| 225268 | pCMV-MCS-P2A-mCherry-N1 | Vector with a multiple cloning site, expressing a P2A ribosome skip sequence and mCherry |
| 225270 | pCMV-MCS-P2A-meGFP(omp25mts) | Vector with a multiple cloning site, a P2A ribosome skip sequence and a mitochondria targeted mEGFP |
| 228545 | 2X-FKBP-meGFP-OMP25mts | FKBP domain targeted to the mitochondrial OMM. Use for rapalog CID system |
| 228547 | pLVX-hSYN-MYC-TRAK1 | MYC-tagged hTRAK1 expressed from the hSYN1 promoter. |