Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding.

Wu JW, Yang JM, Chen CC, Au G, Wang S, Xu Y, Chern GW, Huang CH
iScience. 2025 Oct 11;28(11):113743. doi: 10.1016/j.isci.2025.113743. eCollection 2025 Nov 21. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
254590	FRET-OFF	Engineered low-FRET standard for FRET ratio calibration
254591	FRET-ON	Engineered high-FRET standard for FRET ratio calibration
254592	EKAR1	ERK activity reporter with ECFP-YPet FRET pair