Structural snapshots capture nucleotide release at the mu-opioid receptor.
Khan S, Tyson AS, Ranjbar M, Zhang Z, Singh J, Han GW, Gati C
Nature. 2025 Dec;648(8094):755-763. doi: 10.1038/s41586-025-09677-6. Epub 2025 Nov 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254716 | pcDNA3.1-hMOR-rLuc8 | Expresses human wildtype mu opioid receptor (MOR) with C-terminal rLuc8. Vector used for G protein recruitment or 'nuc-BRET' assay. |
| 254717 | pcDNA3.1-hKOR-rLuc8 | Expresses human wildtype kappa opioid receptor (KOR) with C-terminal rLuc8. Vector used for G protein recruitment or 'nuc-BRET' assay. |
| 254718 | pcDNA3.1-hDOR-rLuc8 | Expresses human wildtype delta opioid receptor (DOR) with C-terminal rLuc8. Vector used for G protein recruitment or 'nuc-BRET' assay. |
| 254719 | pcDNA3.1-Gαi1-Gβ1-Gγ2-GFP2 | Expresses G protein heterotrimer with Gαi1, Gβ1 and Gγ2-GFP2 fusion. Vector used for G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048). |
| 254720 | pcDNA3.1-Gαi1-Gβ1-Gγ2 | Expresses G protein heterotrimer with Gαi1, Gβ1 and Gγ2 ('dark'). Vector used as control for G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048). |
| 254721 | pcDNA3.1-Gαi1ΔF-Gβ1-Gγ2 | Expresses G protein heterotrimer with Gαi1-ΔF, Gβ1 and Gγ2-GFP2 fusion as negative control. Vector used as controlfor G protein recruitment or 'nuc-BRET' assay. Based on TRUPATH Triple Gαi1 (#196048). |