Sensing chromosome bi-orientation by spatial separation of aurora B kinase from kinetochore substrates.
Liu D, Vader G, Vromans MJ, Lampson MA, Lens SM
Science. 2009 Mar 6;323(5919):1350-3. doi: 10.1126/science.1167000. Epub 2009 Jan 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 45227 | Cenp-B INCENP Δcen mCherry | |
| 45228 | Mis12 INCENP Δcen mCherry | |
| 45229 | nonphosphorylatable Mis12-targeted Aurora B FRET sensor | |
| 45230 | nonphosphorylatable Cenp-B-targted Aurora B FRET sensor | |
| 45231 | Mis12-targted Aurora B FRET sensor | |
| 45232 | Cenp-B-targeted Aurora B FRET sensor | |
| 108474 | pCR3-vsv-CenpB 1-166 | expression of vsv-CenpB 1-166 |
| 108475 | GFP-CenpB 1-166 | expression of GFP-CenpB 1-166 |
| 108477 | pCR3-vsv-Mis12-dCEN-Incenp | expression of vsv-Mis12-dCEN-INCENP |
| 108478 | pEGFP-vsv-Mis12-dCEN-Incenp-EGFP | expression of vsv-Mis12-dCEN-INCENP-EGFP |
| 108480 | pcDNA4-Tet-on-vsv-Incenp | inducible expression of vsv-INCENP wt |
| 108481 | pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp | inducible expression of vsv-CenpB-1-166-dCEN-INCENP |
| 108482 | pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp | inducible expression of vsv-Mis12-dCEN-INCENP |
| 108483 | pcDNA4-Tet-on-vsv-Incenp-EGFP | inducible expression of vsv-INCENP wt-EGFP |
| 108484 | pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP | inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP |
| 108485 | pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP | inducible expression of vsv-Mis12-dCEN-INCENP-EGFP |
| 108486 | pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP | inducible expression of vsv-Mis12-dCEN-INCENP-EGFP |
| 108487 | mCherry-Incenp wt | expression of vsv-INCENP wt-mCherry |