Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas.
Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
Genetics. 2013 Aug 26.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47911 | SP6-hCas9-Ce-mRNA | In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase. |
| 47912 | SP6-sgRNA-scaffold | Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection |