DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.
Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP
Nucleic Acids Res. 2013 Dec 4.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51545 | p2attNG-H11-long | p2attNG landing pad plasmid with 5 and 3kb homology arms to the human H11 locus. |
| 51546 | p2attNG-H11-short | p2attNG landing pad plasmid with 400 bp homology arms to the human H11 locus (for use with TALEN-assisted recombination). |
| 51547 | p2attPC | DICE donor plasmid without neural transcription factors. |
| 51551 | p2attPC-FO | DICE donor plasmid with the FOXA2 and OTX2 transcription factors. |
| 51552 | pCMV-Bx | Expresses Bxb1 integrase in mammalian cells. |
| 51553 | pCS-kI | Mammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired. |
| 51554 | MR015-H11-L2-TALEN | Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus. |
| 51555 | MR015-H11-R2-TALEN | Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554). |