The production of viral vectors designed to express large and difficult to express transgenes within neurons.
Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7. PubMed Article
Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62909 | pJEP224-AAV-Basic-0.4αCaMKII-GFP-pA | AAV basic vector backbone designed to express GFP from a 0.4CaMKIIa promoter. It also contains a poly-Adenylation Signal (pA). |
| 62913 | pJEP223-AAV-EFS-GFP-pA | AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA) |
| 62916 | pJEP222-AAV-CMV-GFP-pA | AAV vector backbone designed to express GFP from a CMV promoter. It also contains a poly-Adenylation Signal (pA) |
| 62919 | pJEP221-AAV-RSV-GFP-pA | AAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA) |
| 62920 | pJEP220-AAV-0.5Synapsin-GFP-pA | AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA) |
| 62921 | pJEP219-AAV-1.1Synapsin-GFP-pA | AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA) |
| 62922 | pJEP218-AAV-1.3αCaMKII-GFP-pA | AAV vector backbone designed to express GFP from a 1.3CaMKIIa promoter. |
| 62925 | pJEP217-Lenti-CMV-GFP-WPRE-pA | pLenti vector backbone designed to express GFP from a CMV promoter. |
| 62926 | pJEP216-Lenti-1.3CaMKIIa P-GFP-WPRE-pA | pLenti vector backbone designed to express GFP from a 1.3CaMKIIa promoter. |
| 62927 | pJEP215-Lenti-0.4CaMKIIa P-GFP-WPRE-pA | pLenti vector backbone designed to express GFP from a 0.4CaMKIIa promoter. |
| 62929 | pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA | pLenti vector backbone designed to express GFP from a 0.5Synapsin promoter. |
| 62930 | pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA | pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter . |
| 62931 | pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA | pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation). |
| 63083 | pJEP211-Lenti- (MCS)-WPRE-pA | plenti vector containing MCS-WPRE-pA. Contains no promoter. |
| 63084 | pJEP210-AAV-0.4CaMKII P-MCS-pA | AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA) |
| 74714 | pJEP209-Lenti-1.3CaMKII P-Intron-Flag-GluN2A-WPRE-pA | pLenti vector backbone designed to express Flag-GluN2A from a 1.3CaMKII promoter. Transgene in reverse orientation. |
| 78318 | pJEP207-AAV-CMV-MCS-pA | AAV vector backbone containing a CMV promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA) |
| 78319 | pJEP206-AAV-1.3CaMKII-MCS-pA. | AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA) |
| 84274 | pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA | pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter. |
| 84275 | pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA | pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter. |
| 84276 | pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA | pLenti vector backbone designed to express GFP tagged GluN2A from a CMV promoter. |
| 84277 | pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA | pLenti vector backbone designed to express GFP tagged GluN2B from a CMV promoter. |
| 164724 | pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA | pLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter. |
| 164775 | pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA | pLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter. |
| 164776 | pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA | pLenti vector backbone designed to express GFP-GluN2A from a TRE3G promoter. |
| 164787 | pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA | pLenti vector backbone designed to express GFP-GluN2B from a TRE3G promoter. |
| 164788 | pJEP204-Lenti-TRE3G-GFP-WPRE-Pa | pLenti vector backbone designed to express GFP from a TRE3G promoter. |
| 164789 | pJEP205-AAV-TRE3G-GFP-pA | AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA) |
| 164794 | pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA | pLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter. |
| 164795 | pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA | pLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter. |
| 164796 | pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA | pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter. |