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CRISPR Plasmids: Interfere

Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription. This interference is most efficient when the gRNA targets your gene of interest’s promoter, enhancer, or the beginning of the coding sequence.

dCas9 is shown without a repressor or bound to a repressor. Addition of a gRNA allows the dCas9 to be bound to a target sequence, often located within a promoter region. This binding prevents transcription of the gene.
Figure 1: Overview of CRISPR interference.

Browse, sort, or search the tables below for CRISPR interference plasmids. To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide.

Mammalian

ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication

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Bacteria

ID Plasmid Gene/Insert Promoter PI Publication

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Plant

ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication

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Yeast

ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication

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CRISPR Resources

Addgene has a large selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page. Find a comprehensive list of CRISPR resources by visiting our CRISPR reference page.

Content last reviewed: 17 October 2025

Do you have suggestions for other plasmids that should be added to this list?

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