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Botman-Teusink Yeast FP Collection

Image shows logo of Botman-Teusink Yeast Fluorescent Protein Collection. Four yeast cells each colored in green, red, yellow, and blue are seen on top of the letters B and T.

This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection can be found in Botman et al., 2019.

FP Tagging

Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are available with URA3, SpHIS5 and KANMX markers.

The FP together with the protothropic cassette can be amplified with the following primers:

  • FW 5'–GGTGACGGTGCTGGTTTA–3'
  • RV 5'–TCGATGAATTCGAGCTCG–3'
ID Plasmid Selectable Marker Tags Publication

FP Overexpression

Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and the URA3 marker, the p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter and the LEU2 marker, or the pCEV-G2-Km plasmid (Vickers et al., 2013) containing the TEF1 or PGK1 promoters and the KanMX marker.

ID Plasmid Gene/Insert Selectable Marker Publication

Additional Resources

Addgene Kits

Other Addgene Plasmids

External Resources

References

Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9(1):2234. https://doi.org/10.1038/s41598-019-38913-z (Link opens in a new window) PMID: 30783202 (Link opens in a new window)

Lee, S., Lim, W. A., Thor,n K. S. (2013) Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8(7):e67902. https://doi.org/10.1371/journal.pone.0067902 (Link opens in a new window) PMID: 23844123 (Link opens in a new window)

Loqué, D., Lalonde, S., Looger, L. L., von Wirén, N., Frommer, W. B. (2007). A cytosolic trans-activation domain essential for ammonium uptake. Nature, 446(7132):195-8. https://doi.org/10.1038/nature05579 (Link opens in a new window) PMID: 17293878 (Link opens in a new window)

Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21(8):661-70. https://doi.org/10.1002/yea.1130 (Link opens in a new window) PMID: 15197731 (Link opens in a new window)

Vickers, C. E., Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12:96. https://doi.org/10.1186/1475-2859-12-96 (Link opens in a new window) PMID: 24161108 (Link opens in a new window)

Content last reviewed: 12 August 2025