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Multiplex Yeast Toolkit (MYT)
(Kit # 1000000229 )

Depositing Lab:   Tom Ellis

The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids which extend the capabilities of the MoClo Yeast Toolkit (YTK, Addgene Kit #1000000061) for genetically engineering the yeast, Saccharomyces cerevisiae.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$ 400 USD + shipping

Available to academics and nonprofits only.

Original Publication

A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. PubMed (Link opens in a new window) Article (Link opens in a new window).

Description

MYT includes a set of new integration vectors and selectable markers usable across common lab strains, as well as additional assembly cassettes to increase the number of transcription units in multigene constructs, CRISPR-Cas9 tools for highly efficient multiplexed editing, and three orthogonal and inducible promoter systems for conditional programming of gene expression. MYT is fully backward compatible with YTK, using the same bottom-up hierarchical assembly of single and multi-gene constructs, based on the Golden Gate cloning technique. Integration vectors and assembly cassettes are pre-assembled and ready to go, straight out of the kit, for convenience. Additionally, all of the alternative cloning methods included in YTK, such as BioBrick compatibility, are also present in MYT, while simplifying yeast in vivo assembly.

Figure 1: Overview of constructs found in the MYT including plasmids for genome integration, multiplex CRISPR-Cas9, transcriptional units, and conditional gene expression.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Multiplex Yeast Toolkit (MYT) was a gift from Tom Ellis (Addgene kit #1000000229).”

For your Reference section:

A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. PubMed (Link opens in a new window) Article (Link opens in a new window).

Multiplex Yeast Toolkit (MYT) - #1000000229

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Chloramphenicol
Ampicillin
Kanamycin
Spectinomycin

Inventory

Well Plasmid Resistance
A / 1 pMYT001
Chloramphenicol
A / 2 pMYT002
Chloramphenicol
A / 3 pMYT003
Chloramphenicol
A / 4 pMYT004
Chloramphenicol
A / 5 pMYT005
Chloramphenicol
A / 6 pMYT006
Chloramphenicol
A / 7 pMYT007
Chloramphenicol
A / 8 pMYT008
Chloramphenicol
A / 9 pMYT009
Chloramphenicol
A / 10 pMYT010
Chloramphenicol
A / 11 pMYT011
Chloramphenicol
A / 12 pMYT012
Chloramphenicol
B / 1 pMYT013
Chloramphenicol
B / 2 pMYT014
Chloramphenicol
B / 3 pMYT015
Chloramphenicol
B / 4 pMYT016
Chloramphenicol
B / 5 pMYT017
Chloramphenicol
B / 6 pMYT018
Chloramphenicol
B / 7 pMYT019
Chloramphenicol
B / 8 pMYT020
Chloramphenicol
B / 9 pMYT021
Chloramphenicol
B / 10 pMYT022
Chloramphenicol
B / 11 pMYT023
Chloramphenicol
B / 12 pMYT024
Chloramphenicol
C / 1 pMYT025
Chloramphenicol
C / 2 pMYT026
Chloramphenicol
C / 3 pMYT027
Chloramphenicol
C / 4 pMYT028
Chloramphenicol
C / 5 pMYT029
Chloramphenicol
C / 6 pMYT030
Chloramphenicol
C / 7 pMYT031
Chloramphenicol
C / 8 pMYT032
Chloramphenicol
C / 9 pMYT033
Chloramphenicol
C / 10 pMYT034
Chloramphenicol
C / 11 pMYT035
Chloramphenicol
C / 12 pMYT036
Chloramphenicol
D / 1 pMYT037
Chloramphenicol
D / 2 pMYT038
Chloramphenicol
D / 3 pMYT039
Ampicillin
D / 4 pMYT040
Ampicillin
D / 5 pMYT041
Ampicillin
D / 6 pMYT042
Ampicillin
D / 7 pMYT043
Ampicillin
D / 8 pMYT044
Ampicillin
D / 9 pMYT045
Ampicillin
D / 10 pMYT046
Ampicillin
D / 11 pMYT047
Ampicillin
D / 12 pMYT048
Ampicillin
E / 1 pMYT049
Ampicillin
E / 2 pMYT050
Ampicillin
E / 3 pMYT051
Ampicillin
E / 4 pMYT052
Ampicillin
E / 5 pMYT053
Ampicillin
E / 6 pMYT054
Ampicillin
E / 7 pMYT055
Ampicillin
E / 8 pMYT056
Ampicillin
E / 9 pMYT057
Ampicillin
E / 10 pMYT058
Ampicillin
E / 11 pMYT059
Ampicillin
E / 12 pMYT060
Ampicillin
F / 1 pMYT061
Ampicillin
F / 2 pMYT062
Ampicillin
F / 3 pMYT063
Ampicillin
F / 4 pMYT064
Ampicillin
F / 5 pMYT065
Ampicillin
F / 6 pMYT066
Ampicillin
F / 7 pMYT067
Ampicillin
F / 8 pMYT068
Ampicillin
F / 9 pMYT069
Ampicillin
F / 10 pMYT070
Ampicillin
F / 11 pMYT071
Ampicillin
F / 12 pMYT072
Ampicillin
G / 1 pMYT073
Ampicillin
G / 2 pMYT074
Ampicillin
G / 3 pMYT075
Kanamycin
G / 4 pMYT076
Kanamycin
G / 5 pMYT077
Kanamycin
G / 6 pMYT078
Kanamycin
G / 7 pMYT079
Kanamycin
G / 8 pMYT080
Kanamycin
G / 9 pMYT081
Kanamycin
G / 10 pMYT082
Kanamycin
G / 11 pMYT083
Kanamycin
G / 12 pMYT084
Kanamycin
H / 1 pMYT085
Kanamycin
H / 2 pMYT086
Kanamycin
H / 3 pMYT087
Kanamycin
H / 4 pMYT088
Kanamycin
H / 5 pMYT089
Kanamycin
H / 6 pMYT090
Kanamycin
H / 7 pMYT091
Kanamycin
H / 8 pMYT092
Kanamycin
H / 9 pMYT093
Kanamycin
H / 10 pMYT094
Kanamycin
H / 11 pMYT095
Spectinomycin
H / 12 pMYT096
Spectinomycin
Data calculated @ 2024-04-16

Kit Plate Map - #1000000229

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