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EasyClone 2.0 Yeast Toolkit
(Kit # 1000000073 )

Depositing Lab:   Irina Borodina

EasyClone 2.0 is an expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$330 USD + shipping

Available to academics and nonprofits only.

Original Publication

EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. Stovicek V, Borja GM, Forster J, Borodina I. J Ind Microbiol Biotechnol. 2015 Nov;42(11):1519-31. doi: 10.1007/s10295-015-1684-8. Epub 2015 Sep 16. PubMed .

Description

This collection contains a set of 25 integrative vectors suitable for (over-) expression of (heterologous) genes in both laboratory and industrial S. cerevisiae strains. The vectors allow for selection in auxotrophic yeast strains (four different auxotrophic markers) as well as in prototrophic yeast strains (six different dominant selection markers). The EasyClone system provides a possibility of cloning of up to two genes, when a bidirectional promoter (of any of choice) is used and integration of the construct into well-defined 11 yeast chromosomal locations. Particular biobricks are assembled and cloned into the vectors via Uracil-Specific Excision Reaction (USER) cloning. The marker genes are flanked with loxP sites allowing for marker rescue and recycling, providing a possibility of repeated rounds of gene insertions.

Borodina EasyClone kit vector map

Depositor-provided Files

Kit Manual: Icon Borodina EasyClone 2.0 User Manual (1.2 MB)

Additional References

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The EasyClone 2.0 kit was a gift from Irina Borodina (Addgene kit # 1000000073 ).”

For your Reference section:

EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. Stovicek V, Borja GM, Forster J, Borodina I. J Ind Microbiol Biotechnol. 2015 Nov;42(11):1519-31. doi: 10.1007/s10295-015-1684-8. Epub 2015 Sep 16. PubMed .

EasyClone 2.0 Yeast Toolkit - #1000000073

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pCfB2188
Ampicillin
A / 2 pCfB2189
Ampicillin
A / 3 pCfB2226
Ampicillin
A / 4 pCfB2374
Ampicillin
A / 5 pCfB2190
Ampicillin
A / 6 pCfB2227
Ampicillin
A / 7 pCfB2229
Ampicillin
A / 8 pCfB2191
Ampicillin
A / 9 pCfB2192
Ampicillin
A / 10 pCfB2228
Ampicillin
A / 11 pCfB2336
Ampicillin
A / 12 pCfB2193
Ampicillin
B / 1 pCfB2513
Ampicillin
B / 2 pCfB2223
Ampicillin
B / 3 pCfB2194
Ampicillin
B / 4 pCfB2375
Ampicillin
B / 5 pCfB2224
Ampicillin
B / 6 pCfB2195
Ampicillin
B / 7 pCfB2196
Ampicillin
B / 8 pCfB2399
Ampicillin
B / 9 pCfB2197
Ampicillin
B / 10 pCfB2400
Ampicillin
B / 11 pCfB2225
Ampicillin
B / 12 pCfB2198
Ampicillin
C / 1 pCfB2337
Ampicillin
Data calculated @ 2017-05-25

Kit Plate Map - #1000000073

Plate #1

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A
B
C
D
E
F
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H
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