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Alexandrov Cell-Free Protein Expression Kit (PEK)
(Kit # 1000000065 )

Depositing Lab:   Kirill Alexandrov

This collection contains a set of 91 plasmids: 2 empty backbone control plasmids (pLTE EGFP and pCellFree_G03) and 89 open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$175 USD + shipping

Available to academics and nonprofits only.

Original Publication

Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov Küth FX. Biotechnol Bioeng 2015 Aug 24. doi: 10.1002/bit.25814. PubMed .

Description

The Alexandrov Cell-Free Protein Expression kit represents a set of 89 open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system. The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems. The set of proteins encoded is one of the most popular ones in the biomedical research community and range in size form 4 to 156 kDa, which enables the user to characterize the efficiency of their cell-free system in correlation to the size of the product. The analysis can be conveniently performed using the N-terminal eGFP tag carried by all constructs.

Please note that Well-G6 is a duplicate of Well-G5. Both Wells contain plasmid pCellFree_G03 STAT3.

Kit Documentation

Documents included with shipment: Icon alexandrov-cell-free-PEK.pdf (76.3 KB)

Additional Reference

The pCellFree_G03 plasmid provided with this kit was generated in a different publication. Please, cite the following reference when using this plasmid:

Gateway-compatible vectors for high-throughput protein expression in pro- and eukaryotic cell-free systems. Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Škalamera D, Gonda TJ, Alexandrov K. J Biotechnol 2015 Feb 10;195:1-7. doi: 10.1016/j.jbiotec.2014.12.006. PubMed .

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Alexandrov Cell-Free Protein Expression Kit was a gift from Kirill Alexandrov (Addgene kit # 1000000065).”

For your Reference section:

Performance benchmarking of four cell-free protein expression systems. Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov Küth FX. Biotechnol Bioeng 2015 Aug 24. doi: 10.1002/bit.25814. PubMed .

Alexandrov Cell-Free Protein Expression Kit (PEK) - #1000000065

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Chloramphenicol and Ampicillin

Inventory

Well Plasmid Resistance
A / 1 pLTE EGFP
Ampicillin
A / 2 pCellFree_G05 sfGFP
Ampicillin
A / 3 pCellFree_G03 E-Cad
Ampicillin
A / 4 pCellFree_G03 TFF1
Ampicillin
A / 5 pCellFree_G03 S100A1
Ampicillin
A / 6 pCellFree_G03 TCEB2
Ampicillin
A / 7 pCellFree_G03 BATF3
Ampicillin
A / 8 pCellFree_G03 BTF3L4
Ampicillin
A / 9 pCellFree_G03 VPS29
Ampicillin
A / 10 pCellFree_G03 IL2
Ampicillin
A / 11 pCellFree_G03 ISG15
Ampicillin
A / 12 pCellFree_G03 CIRBP
Ampicillin
B / 1 pCellFree_G03 IFNG
Ampicillin
B / 2 pCellFree_G03 ATF3
Ampicillin
B / 3 pCellFree_G03 RAP2A
Ampicillin
B / 4 pCellFree_G03 BTF3
Ampicillin
B / 5 pCellFree_G03 TFEC
Ampicillin
B / 6 pCellFree_G03 IL6
Ampicillin
B / 7 pCellFree_G03 TNF
Ampicillin
B / 8 pCellFree_G03 YWHAB
Ampicillin
B / 9 pCellFree_G03 TFAM
Ampicillin
B / 10 pCellFree_G03 ATF1
Ampicillin
B / 11 pCellFree_G03 IL1B
Ampicillin
B / 12 pCellFree_G03 GTF2B
Ampicillin
C / 1 pCellFree_G03 GATA1
Ampicillin
C / 2 pCellFree_G03 USF2
Ampicillin
C / 3 pCellFree_G03 CREBZF
Ampicillin
C / 4 pCellFree_G03 ANXA1
Ampicillin
C / 5 pCellFree_G03 EBNA1BP2
Ampicillin
C / 6 pCellFree_G03 TFAP4
Ampicillin
C / 7 pCellFree_G03 SP6
Ampicillin
C / 8 pCellFree_G03 CAMK1
Ampicillin
C / 9 pCellFree_G03 GTF2A1
Ampicillin
C / 10 pCellFree_G03 MAPK13
Ampicillin
C / 11 pCellFree_G03 TP53
Ampicillin
C / 12 pCellFree_G03 IRF9
Ampicillin
D / 1 pCellFree_G03 TGFB1
Ampicillin
D / 2 pCellFree_G03 GTF2H2D
Ampicillin
D / 3 pCellFree_G03 YY1
Ampicillin
D / 4 pCellFree_G03 TFDP3
Ampicillin
D / 5 pCellFree_G03 TFDP1
Ampicillin
D / 6 pCellFree_G03 CSNK2A1
Ampicillin
D / 7 pCellFree_G03 CALR
Ampicillin
D / 8 pCellFree_G03 PSMC1
Ampicillin
D / 9 pCellFree_G03 TFDP2
Ampicillin
D / 10 pCellFree_G03 GTF2E1
Ampicillin
D / 11 pCellFree_G03 CYP2D6
Ampicillin
D / 12 pCellFree_G03 RUVBL1
Ampicillin
E / 1 pCellFree_G03 TFAP2B
Ampicillin
E / 2 pCellFree_G03 GDI2
Ampicillin
E / 3 pCellFree_G03 GTF2H4
Ampicillin
E / 4 pCellFree_G03 SMAD2
Ampicillin
E / 5 pCellFree_G03 GTF2A1L
Ampicillin
E / 6 pCellFree_G03 TFEB
Ampicillin
E / 7 pCellFree_G03 WAVE 2
Ampicillin
E / 8 pCellFree_G03 PPARG
Ampicillin
E / 9 pCellFree_G03 CTTN
Ampicillin
E / 10 pCellFree_G03 HDAC1
Ampicillin
E / 11 pCellFree_G03 AKT1
Ampicillin
E / 12 pCellFree_G03 CYP2A13
Ampicillin
F / 1 pCellFree_G03 CYP3A4
Ampicillin
F / 2 pCellFree_G03 BMPR1A
Ampicillin
F / 3 pCellFree_G03 FYN
Ampicillin
F / 4 pCellFree_G03 EHD2
Ampicillin
F / 5 pCellFree_G03 GTF2H1
Ampicillin
F / 6 pCellFree_G03 SP2
Ampicillin
F / 7 pCellFree_G03 TCF3
Ampicillin
F / 8 pCellFree_G03 DDX5
Ampicillin
F / 9 pCellFree_G03 RRN3
Ampicillin
F / 10 pCellFree_G03 TCF12
Ampicillin
F / 11 pCellFree_G03 TCF25
Ampicillin
F / 12 pCellFree_G03 PRKCA
Ampicillin
G / 1 pCellFree_G03 STAT1
Ampicillin
G / 2 pCellFree_G03 TCEB3B
Ampicillin
G / 3 pCellFree_G03 FOXM
Ampicillin
G / 4 pCellFree_G03 CTNNB1
Ampicillin
G / 5 pCellFree_G03 STAT3
Ampicillin
G / 6 pCellFree_G03 STAT3
Ampicillin
G / 7 pCellFree_G03 STAT6
Ampicillin
G / 8 pCellFree_G03 RAD54B
Ampicillin
G / 9 pCellFree_G03 WWP1
Ampicillin
G / 10 pCellFree_G03 NFKB1
Ampicillin
G / 11 pCellFree_G03 BMPR2
Ampicillin
G / 12 pCellFree_G03 POLD1
Ampicillin
H / 1 pCellFree_G03 SREBF2
Ampicillin
H / 2 pCellFree_G03 PDGFRB
Ampicillin
H / 3 pCellFree_G03 NFX1
Ampicillin
H / 4 pCellFree_G03 PC
Ampicillin
H / 5 pCellFree_G03 TYK2
Ampicillin
H / 6 pCellFree_G03 ZFYVE9
Ampicillin
H / 7 pCellFree_G03
Chloramphenicol and Ampicillin
Data calculated @ 2017-05-25

Kit Plate Map - #1000000065

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