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Vaccinia Virus ORF Library
(Kit # 1000000009 )

Depositing Labs:   Jack Bennink, Jon Yewdell

This is a vaccinia virus (VACV) expression library containing each of the predicted open reading frames.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$800 USD + shipping

Available to academics and nonprofits only.

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Original Publication

Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines. Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW. J Exp Med. 2005 Jan 3. 201(1):95-104. (PubMed PMID: 15623576).

Description

This collection of vaccinia virus (VACV) ORF clones was made using re-amplified PCR products generated originally for integration into yeast for a 2-hybrid project: McCraith S, Holtzman T, Moss B, and Fields S. (2000). Genome-wide analysis of vaccinia virus protein-protein interactions. Proc. Natl. Acad. Sci. USA 97: 4879-84.

The ORF definition for the original 2-hybrid project was based on an unpublished sequence of vaccinia virus strain WR in collaboration with Dr Bernard Moss at NIAID, NIH, Bethesda, MD.

The spreadsheet listing all the ORFs includes 1) an ORF name that relates only to this library and no published genome annotation, 2) the name of the ORF (if present) in VACV strain Copenhagen (GenBank accession M35027) and 3) the name of the ORF (if annotated) in VACV strain WR sequence (GenBank accession AY243312), which was published after these clones were made.

There are some differences between the ORFs defined by this collection of clones and the ORFs annotated on the published VACV WR sequence. Some arise from sequence errors in the unpublished sequence, others from differences in annotation. Please note that the amino acid sequence given for each ORF in the spreadsheet is derived from the unpublished sequence and has some errors.

All ORFs retain the native stop codon and nearly all have a common sequence added to each end that allowed all ORFs in the original set to be re-amplified by PCR using a single primer pair. These common sequences are: 5' end: CGAATTCCAGCTGACCACC and 3' end: CGGATCCCCGGGAATTGC. NB: some ORFs were cloned directly from VACV genomic DNA for my project and lack these common sequences, these are VVorfs 8, 27, 30, 31, 107, 151, 243.

For all ORFs flanked by the common sequences, Bam HI excises a product that is roughly the size of the insert (unless there are internal BamHI sites); there is a Bam HI site in the 3' common sequence and one just 5' of the TOPO insertion site in the vector.

Up to roughly 500 bp from the 5’ end of most clones was sequenced and many have base changes within the sequenced region (primer and PCR fidelity errors). The extent of my sequencing and any clones with known errors are the last 2 columns of the spreadsheet.

No attempt has been made to test how many of these ORFs express a protein after transfection into mammalian cells.

Given all the above, the clone collection remains a useful (but by no means ideal) set for expression screening and should not be relied on as a complete source of error-free ORFs.

Documentation

Icon VVOrfData.tab.xls (91.4 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The Vaccinia Virus plasmids were a gift from Jack Bennink and Jon Yewdell (Addgene Kit # 1000000009)"

For your Reference section:

Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines. Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW. J Exp Med. 2005 Jan 3. 201(1):95-104. (PubMed PMID: 15623576).

Vaccinia Virus ORF Library - #1000000009

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 DCT.VV.Orf1
Ampicillin
Plate 1 / A / 2 DCT.VV.Orf2
Ampicillin
Plate 1 / A / 3 DCT.VV.Orf3
Ampicillin
Plate 1 / A / 4 DCT.VV.Orf4
Ampicillin
Plate 1 / A / 5 DCT.VV.Orf5
Ampicillin
Plate 1 / A / 6 DCT.VV.Orf6
Ampicillin
Plate 1 / A / 7 DCT.VV.Orf7
Ampicillin
Plate 1 / A / 8 DCT.VV.Orf8
Ampicillin
Plate 1 / A / 9 DCT.VV.Orf9
Ampicillin
Plate 1 / A / 10 DCT.VV.Orf10
Ampicillin
Plate 1 / A / 11 DCT.VV.Orf11
Ampicillin
Plate 1 / A / 12 DCT.VV.Orf12
Ampicillin
Plate 1 / B / 1 DCT.VV.Orf13
Ampicillin
Plate 1 / B / 2 DCT.VV.Orf15
Ampicillin
Plate 1 / B / 3 DCT.VV.Orf16
Ampicillin
Plate 1 / B / 4 DCT.VV.Orf17
Ampicillin
Plate 1 / B / 5 DCT.VV.Orf18
Ampicillin
Plate 1 / B / 6 DCT.VV.Orf19
Ampicillin
Plate 1 / B / 7 DCT.VV.Orf20
Ampicillin
Plate 1 / B / 8 DCT.VV.Orf21
Ampicillin
Plate 1 / B / 9 DCT.VV.Orf22
Ampicillin
Plate 1 / B / 10 DCT.VV.Orf23
Ampicillin
Plate 1 / B / 11 DCT.VV.Orf25
Ampicillin
Plate 1 / B / 12 DCT.VV.Orf26
Ampicillin
Plate 1 / C / 1 DCT.VV.Orf27
Ampicillin
Plate 1 / C / 2 DCT.VV.Orf28
Ampicillin
Plate 1 / C / 3 DCT.VV.Orf29
Ampicillin
Plate 1 / C / 4 DCT.VV.Orf30
Ampicillin
Plate 1 / C / 5 DCT.VV.Orf31
Ampicillin
Plate 1 / C / 6 DCT.VV.Orf32
Ampicillin
Plate 1 / C / 7 DCT.VV.Orf33
Ampicillin
Plate 1 / C / 8 DCT.VV.Orf34
Ampicillin
Plate 1 / C / 9 DCT.VV.Orf35
Ampicillin
Plate 1 / C / 10 DCT.VV.Orf36
Ampicillin
Plate 1 / C / 11 DCT.VV.Orf37
Ampicillin
Plate 1 / C / 12 DCT.VV.Orf38
Ampicillin
Plate 1 / D / 1 DCT.VV.Orf39
Ampicillin
Plate 1 / D / 2 DCT.VV.Orf40
Ampicillin
Plate 1 / D / 3 DCT.VV.Orf41
Ampicillin
Plate 1 / D / 4 DCT.VV.Orf42
Ampicillin
Plate 1 / D / 5 DCT.VV.Orf43
Ampicillin
Plate 1 / D / 6 DCT.VV.Orf44
Ampicillin
Plate 1 / D / 7 DCT.VV.Orf45
Ampicillin
Plate 1 / D / 8 DCT.VV.Orf46
Ampicillin
Plate 1 / D / 9 DCT.VV.Orf47
Ampicillin
Plate 1 / D / 10 DCT.VV.Orf48
Ampicillin
Plate 1 / D / 11 DCT.VV.Orf49
Ampicillin
Plate 1 / D / 12 DCT.VV.Orf50
Ampicillin
Plate 1 / E / 1 DCT.VV.Orf51
Ampicillin
Plate 1 / E / 2 DCT.VV.Orf52
Ampicillin
Plate 1 / E / 3 DCT.VV.Orf53
Ampicillin
Plate 1 / E / 4 DCT.VV.Orf54
Ampicillin
Plate 1 / E / 5 DCT.VV.Orf55
Ampicillin
Plate 1 / E / 6 DCT.VV.Orf56
Ampicillin
Plate 1 / E / 7 DCT.VV.Orf57
Ampicillin
Plate 1 / E / 8 DCT.VV.Orf58
Ampicillin
Plate 1 / E / 9 DCT.VV.Orf59
Ampicillin
Plate 1 / E / 10 DCT.VV.Orf60
Ampicillin
Plate 1 / E / 11 DCT.VV.Orf61
Ampicillin
Plate 1 / E / 12 DCT.VV.Orf62
Ampicillin
Plate 1 / F / 1 DCT.VV.Orf63
Ampicillin
Plate 1 / F / 2 DCT.VV.Orf64
Ampicillin
Plate 1 / F / 3 DCT.VV.Orf65
Ampicillin
Plate 1 / F / 4 DCT.VV.Orf66
Ampicillin
Plate 1 / F / 5 DCT.VV.Orf67
Ampicillin
Plate 1 / F / 6 DCT.VV.Orf68
Ampicillin
Plate 1 / F / 7 DCT.VV.Orf69
Ampicillin
Plate 1 / F / 8 DCT.VV.Orf70
Ampicillin
Plate 1 / F / 9 DCT.VV.Orf71
Ampicillin
Plate 1 / F / 10 DCT.VV.Orf72
Ampicillin
Plate 1 / F / 11 DCT.VV.Orf73
Ampicillin
Plate 1 / F / 12 DCT.VV.Orf74
Ampicillin
Plate 1 / G / 1 DCT.VV.Orf75
Ampicillin
Plate 1 / G / 2 DCT.VV.Orf76
Ampicillin
Plate 1 / G / 3 DCT.VV.Orf77
Ampicillin
Plate 1 / G / 4 DCT.VV.Orf78
Ampicillin
Plate 1 / G / 5 DCT.VV.Orf79
Ampicillin
Plate 1 / G / 6 DCT.VV.Orf80
Ampicillin
Plate 1 / G / 7 DCT.VV.Orf81
Ampicillin
Plate 1 / G / 8 DCT.VV.Orf82
Ampicillin
Plate 1 / G / 9 DCT.VV.Orf83
Ampicillin
Plate 1 / G / 10 DCT.VV.Orf84
Ampicillin
Plate 1 / G / 11 DCT.VV.Orf85
Ampicillin
Plate 1 / G / 12 DCT.VV.Orf86
Ampicillin
Plate 1 / H / 1 DCT.VV.Orf87
Ampicillin
Plate 1 / H / 2 DCT.VV.Orf88
Ampicillin
Plate 1 / H / 3 DCT.VV.Orf89
Ampicillin
Plate 1 / H / 4 DCT.VV.Orf90
Ampicillin
Plate 1 / H / 5 DCT.VV.Orf91
Ampicillin
Plate 1 / H / 6 DCT.VV.Orf92
Ampicillin
Plate 1 / H / 7 DCT.VV.Orf93
Ampicillin
Plate 1 / H / 8 DCT.VV.Orf94
Ampicillin
Plate 1 / H / 9 DCT.VV.Orf95
Ampicillin
Plate 1 / H / 10 DCT.VV.Orf96
Ampicillin
Plate 1 / H / 11 DCT.VV.Orf97
Ampicillin
Plate 1 / H / 12 DCT.VV.Orf98
Ampicillin
Plate 2 / A / 1 DCT.VV.Orf99
Ampicillin
Plate 2 / A / 2 DCT.VV.Orf100
Ampicillin
Plate 2 / A / 3 DCT.VV.Orf101
Ampicillin
Plate 2 / A / 4 DCT.VV.Orf102
Ampicillin
Plate 2 / A / 5 DCT.VV.Orf103
Ampicillin
Plate 2 / A / 6 DCT.VV.Orf104
Ampicillin
Plate 2 / A / 7 DCT.VV.Orf105
Ampicillin
Plate 2 / A / 8 DCT.VV.Orf106
Ampicillin
Plate 2 / A / 9 DCT.VV.Orf107
Ampicillin
Plate 2 / A / 10 DCT.VV.Orf108
Ampicillin
Plate 2 / A / 11 DCT.VV.Orf109
Ampicillin
Plate 2 / A / 12 DCT.VV.Orf110
Ampicillin
Plate 2 / B / 1 DCT.VV.Orf111
Ampicillin
Plate 2 / B / 2 DCT.VV.Orf112
Ampicillin
Plate 2 / B / 3 DCT.VV.Orf113
Ampicillin
Plate 2 / B / 4 DCT.VV.Orf114
Ampicillin
Plate 2 / B / 5 DCT.VV.Orf115
Ampicillin
Plate 2 / B / 6 DCT.VV.Orf116
Ampicillin
Plate 2 / B / 7 DCT.VV.Orf117
Ampicillin
Plate 2 / B / 8 DCT.VV.Orf118
Ampicillin
Plate 2 / B / 9 DCT.VV.Orf125
Ampicillin
Plate 2 / B / 10 DCT.VV.Orf126
Ampicillin
Plate 2 / B / 11 DCT.VV.Orf127
Ampicillin
Plate 2 / B / 12 DCT.VV.Orf128
Ampicillin
Plate 2 / C / 1 DCT.VV.Orf129
Ampicillin
Plate 2 / C / 2 DCT.VV.Orf130
Ampicillin
Plate 2 / C / 3 DCT.VV.Orf131
Ampicillin
Plate 2 / C / 4 DCT.VV.Orf132
Ampicillin
Plate 2 / C / 5 DCT.VV.Orf133
Ampicillin
Plate 2 / C / 6 DCT.VV.Orf134
Ampicillin
Plate 2 / C / 7 DCT.VV.Orf135
Ampicillin
Plate 2 / C / 8 DCT.VV.Orf136
Ampicillin
Plate 2 / C / 9 DCT.VV.Orf137
Ampicillin
Plate 2 / C / 10 DCT.VV.Orf138
Ampicillin
Plate 2 / C / 11 DCT.VV.Orf139
Ampicillin
Plate 2 / C / 12 DCT.VV.Orf140
Ampicillin
Plate 2 / D / 1 DCT.VV.Orf141
Ampicillin
Plate 2 / D / 2 DCT.VV.Orf142
Ampicillin
Plate 2 / D / 3 DCT.VV.Orf143
Ampicillin
Plate 2 / D / 4 DCT.VV.Orf144
Ampicillin
Plate 2 / D / 5 DCT.VV.Orf145
Ampicillin
Plate 2 / D / 6 DCT.VV.Orf146
Ampicillin
Plate 2 / D / 7 DCT.VV.Orf147
Ampicillin
Plate 2 / D / 8 DCT.VV.Orf148
Ampicillin
Plate 2 / D / 9 DCT.VV.Orf149
Ampicillin
Plate 2 / D / 10 DCT.VV.Orf150
Ampicillin
Plate 2 / D / 11 DCT.VV.Orf151
Ampicillin
Plate 2 / D / 12 DCT.VV.Orf152
Ampicillin
Plate 2 / E / 1 DCT.VV.Orf153
Ampicillin
Plate 2 / E / 2 DCT.VV.Orf154
Ampicillin
Plate 2 / E / 3 DCT.VV.Orf155
Ampicillin
Plate 2 / E / 4 DCT.VV.Orf156
Ampicillin
Plate 2 / E / 5 DCT.VV.Orf157
Ampicillin
Plate 2 / E / 6 DCT.VV.Orf158
Ampicillin
Plate 2 / E / 7 DCT.VV.Orf159
Ampicillin
Plate 2 / E / 8 DCT.VV.Orf160
Ampicillin
Plate 2 / E / 9 DCT.VV.Orf161
Ampicillin
Plate 2 / E / 10 DCT.VV.Orf162
Ampicillin
Plate 2 / E / 11 DCT.VV.Orf163
Ampicillin
Plate 2 / E / 12 DCT.VV.Orf164
Ampicillin
Plate 2 / F / 1 DCT.VV.Orf165
Ampicillin
Plate 2 / F / 2 DCT.VV.Orf166
Ampicillin
Plate 2 / F / 3 DCT.VV.Orf167
Ampicillin
Plate 2 / F / 4 DCT.VV.Orf168
Ampicillin
Plate 2 / F / 5 DCT.VV.Orf169
Ampicillin
Plate 2 / F / 6 DCT.VV.Orf170
Ampicillin
Plate 2 / F / 7 DCT.VV.Orf171
Ampicillin
Plate 2 / F / 8 DCT.VV.Orf172
Ampicillin
Plate 2 / F / 9 DCT.VV.Orf173
Ampicillin
Plate 2 / F / 10 DCT.VV.Orf174
Ampicillin
Plate 2 / F / 11 DCT.VV.Orf175
Ampicillin
Plate 2 / F / 12 DCT.VV.Orf176
Ampicillin
Plate 2 / G / 1 DCT.VV.Orf177
Ampicillin
Plate 2 / G / 2 DCT.VV.Orf178
Ampicillin
Plate 2 / G / 3 DCT.VV.Orf179
Ampicillin
Plate 2 / G / 4 DCT.VV.Orf180
Ampicillin
Plate 2 / G / 5 DCT.VV.Orf181
Ampicillin
Plate 2 / G / 6 DCT.VV.Orf182
Ampicillin
Plate 2 / G / 7 DCT.VV.Orf183
Ampicillin
Plate 2 / G / 8 DCT.VV.Orf184
Ampicillin
Plate 2 / G / 9 DCT.VV.Orf185
Ampicillin
Plate 2 / G / 10 DCT.VV.Orf186
Ampicillin
Plate 2 / G / 11 DCT.VV.Orf187
Ampicillin
Plate 2 / G / 12 DCT.VV.Orf188
Ampicillin
Plate 2 / H / 1 DCT.VV.Orf189
Ampicillin
Plate 2 / H / 2 DCT.VV.Orf190
Ampicillin
Plate 2 / H / 3 DCT.VV.Orf191
Ampicillin
Plate 2 / H / 4 DCT.VV.Orf192
Ampicillin
Plate 2 / H / 5 DCT.VV.Orf193
Ampicillin
Plate 2 / H / 6 DCT.VV.Orf194
Ampicillin
Plate 2 / H / 7 DCT.VV.Orf195
Ampicillin
Plate 2 / H / 8 DCT.VV.Orf196
Ampicillin
Plate 2 / H / 9 DCT.VV.Orf197
Ampicillin
Plate 2 / H / 10 DCT.VV.Orf198
Ampicillin
Plate 2 / H / 11 DCT.VV.Orf199
Ampicillin
Plate 2 / H / 12 DCT.VV.Orf200
Ampicillin
Plate 3 / A / 1 DCT.VV.Orf201
Ampicillin
Plate 3 / A / 2 DCT.VV.Orf202
Ampicillin
Plate 3 / A / 3 DCT.VV.Orf203
Ampicillin
Plate 3 / A / 4 DCT.VV.Orf204
Ampicillin
Plate 3 / A / 5 DCT.VV.Orf205
Ampicillin
Plate 3 / A / 6 DCT.VV.Orf206
Ampicillin
Plate 3 / A / 7 DCT.VV.Orf207
Ampicillin
Plate 3 / A / 8 DCT.VV.Orf208
Ampicillin
Plate 3 / A / 9 DCT.VV.Orf209
Ampicillin
Plate 3 / A / 10 DCT.VV.Orf210
Ampicillin
Plate 3 / A / 11 DCT.VV.Orf211
Ampicillin
Plate 3 / A / 12 DCT.VV.Orf212
Ampicillin
Plate 3 / B / 1 DCT.VV.Orf213
Ampicillin
Plate 3 / B / 2 DCT.VV.Orf214
Ampicillin
Plate 3 / B / 3 DCT.VV.Orf215
Ampicillin
Plate 3 / B / 4 DCT.VV.Orf216
Ampicillin
Plate 3 / B / 5 DCT.VV.Orf217
Ampicillin
Plate 3 / B / 6 DCT.VV.Orf218
Ampicillin
Plate 3 / B / 7 DCT.VV.Orf219
Ampicillin
Plate 3 / B / 8 DCT.VV.Orf220
Ampicillin
Plate 3 / B / 9 DCT.VV.Orf221
Ampicillin
Plate 3 / B / 10 DCT.VV.Orf222
Ampicillin
Plate 3 / B / 11 DCT.VV.Orf223
Ampicillin
Plate 3 / B / 12 DCT.VV.Orf224
Ampicillin
Plate 3 / C / 1 DCT.VV.Orf225
Ampicillin
Plate 3 / C / 2 DCT.VV.Orf226
Ampicillin
Plate 3 / C / 3 DCT.VV.Orf227
Ampicillin
Plate 3 / C / 4 DCT.VV.Orf228
Ampicillin
Plate 3 / C / 5 DCT.VV.Orf229
Ampicillin
Plate 3 / C / 6 DCT.VV.Orf232
Ampicillin
Plate 3 / C / 7 DCT.VV.Orf233
Ampicillin
Plate 3 / C / 8 DCT.VV.Orf234
Ampicillin
Plate 3 / C / 9 DCT.VV.Orf235
Ampicillin
Plate 3 / C / 10 DCT.VV.Orf236
Ampicillin
Plate 3 / C / 11 DCT.VV.Orf237
Ampicillin
Plate 3 / C / 12 DCT.VV.Orf238
Ampicillin
Plate 3 / D / 1 DCT.VV.Orf239
Ampicillin
Plate 3 / D / 2 DCT.VV.Orf240
Ampicillin
Plate 3 / D / 3 DCT.VV.Orf241
Ampicillin
Plate 3 / D / 4 DCT.VV.Orf242
Ampicillin
Plate 3 / D / 5 DCT.VV.Orf243
Ampicillin
Plate 3 / D / 6 DCT.VV.Orf249
Ampicillin
Plate 3 / D / 7 DCT.VV.Orf250
Ampicillin
Plate 3 / D / 8 DCT.VV.Orf251
Ampicillin
Plate 3 / D / 9 DCT.VV.Orf252
Ampicillin
Plate 3 / D / 10 DCT.VV.Orf253
Ampicillin
Plate 3 / D / 11 DCT.VV.Orf254
Ampicillin
Plate 3 / D / 12 DCT.VV.Orf255
Ampicillin
Plate 3 / E / 1 DCT.VV.Orf256
Ampicillin
Plate 3 / E / 2 DCT.VV.Orf257
Ampicillin
Plate 3 / E / 3 DCT.VV.Orf259
Ampicillin
Plate 3 / E / 4 DCT.VV.Orf260
Ampicillin
Plate 3 / E / 5 DCT.VV.Orf261
Ampicillin
Plate 3 / E / 6 DCT.VV.Orf262
Ampicillin
Plate 3 / E / 7 DCT.VV.Orf263
Ampicillin
Plate 3 / E / 8 DCT.VV.Orf264
Ampicillin
Plate 3 / E / 9 DCT.VV.Orf265
Ampicillin
Plate 3 / E / 10 DCT.VV.Orf266
Ampicillin
Plate 3 / E / 11 DCT.VV.Orf268
Ampicillin
Plate 3 / E / 12 DCT.VV.Orf269
Ampicillin
Plate 3 / F / 1 DCT.VV.Orf270
Ampicillin
Data calculated @ 2017-09-19

Kit Plate Map - #1000000009

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