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pMuSIC pool v1.0
(Pooled Library #227193)

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  • Purpose

    This pool contains 153 unique fluorescent protein barcodes made from 2-way combinations of 18 individual fluorescent proteins. They provide single cell resolution in spectral detection measurements (e.g. spectral flow cytometry). Potential applications include lineage tracing, or with some additional design, pairing with perturbations.

  • Vector Backbone

    pReceiver (Plasmid #228594)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 227193 pMuSIC pool v1.0 1 $380 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Number of Fluorescent Protein Permutations
    324
  • Number of Fluorescent Protein Combinations
    153

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼10 µL
  • Concentration
    15 ng/µL

Resource Information

Depositor Comments

The pMuSIC library includes 153 two-probe barcodes that are generated from 18 fluorescent proteins (fps). These 153 barcodes come from 324 possible permutations of the two fps, as order of the fps does not affect the unique barcode spectra.

The schematic shows the preparation of the pMuSIC pooled library. It is noted that to amplify the library, the purified library should be diluted and re-transformed. To get a representation of 10X for each of the 324 barcodes, you need at least 3240 colonies total. See manuscript for additional details.

Figure 1. Schematic of pMuSIC library design. 18 fluorescent reporters (FPs) were PCR amplified and cloned individually into pR and pM pools using Golden Gate assembly. pR and pM vectors were combined using Golden Gate assembly to make pMuSIC plasmids containing two FPs each. The pMuSIC plasmids were transformed and divided into two plates. On one plate, single colonies (~400) were miniprepped to identify the pMuSIC combinations. On the other plate, all colonies (~2500) were scraped into a single pool and purified. The purified pool was confirmed by Nanopore sequencing.

Please visit https://doi.org/10.1101/2024.10.23.619855 (Link opens in a new window)for bioRxiv preprint.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuSIC pool v1.0 library was a gift from Marc Birtwistle (Addgene #227193)

  • For your References section:

    Genetically-Encoded Fluorescence Barcodes for Single-Cell Analysis. Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye F, Biswas T, Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR. bioRxiv [Preprint]. 2024 Oct 24:2024.10.23.619855. doi: 10.1101/2024.10.23.619855. PubMed 39484616
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