Phage ORFeome Library
(Pooled Library #249629)

• Purpose:

  This library consists of approximately 400 phage open reading frames (ORFs), each cloned into an IPTG-inducible vector. The library can be transformed into bacteria expressing antiphage systems to uncover the triggers responsible for immune activation.

• Vector Backbone:

  pTN1011_pTACxc-MBP-mCherry (GB, 21 KB)

• Depositing Labs: Aaron Whiteley
• Publication: Nagy et al. Nat Microbiol. 2026 Feb;11(2):597-609. doi: 10.1038/s41564-025-02239-6. Epub 2026 Jan 16.

Library Details

• Species: ORFs from the following phages: MS2, T2, T7, λ, M13, and φX174
• Genes targeted: 406
• Size of inserts: ~200–4000 bp

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼15 µL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Amplification Protocol (DOCX, 3.1 MB)
• Depositor Data:
  • Plasmid Maps (ZIP, 2.8 MB)
  • Concatenated Phage Genomes (GB, 824 KB)
  • NGS Reads S159_R1_001 (FASTQ.GZ, 83.3 MB)
  • NGS Reads S159_R2_001 (FASTQ.GZ, 86.4 MB)

Terms and Licenses

Academic/Nonprofit Terms:

• UBMTA

Industry Terms:

• Not Available to Industry

Trademarks:

• Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.02.662641 for bioRxiv preprint.