LentiGuide-Puro-P2A-EGFP_mRFPstuf
Plasmid
#137730
Purpose
Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
Depositor
Type
Empty backbone
Use
CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
Tags
Expression
Bacterial and Mammalian
Mutation
Promoter
Availability
Academic Institutions and Nonprofits only