-
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pBR.DT-A
Plasmid#35955DepositorInsertdiphtheria toxin A
UseBac recombineeringTagsExpressionMutationPromoterPGKAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBRα
Plasmid#53731PurposePlpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertαNTD
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH625_pJH_LldR_PlldR-sfGFP.0TAG
Plasmid#163724PurposeLactate-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertlldr gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Flag
Plasmid#17364DepositorInsertMMLV proviral DNA
UseTagsFlagExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHS
Plasmid#166938PurposeToehold switch multi-level gene expression controller (MLC)DepositorInsertGenetic circuit expressing GFP- Toehold switch
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA (KmR)
Plasmid#102321Purposedrives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)DepositorInsertRpaA with p0050 promoter (+ kanamycin resistance casette)
UseCloning vectorTagsExpressionMutationPromoter-Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Forsur-6
Plasmid#188860PurposeAvs inhibitor Forsur-6DepositorInsertForsur-6
UseTagsExpressionBacterialMutationPromoterJ23105Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lidtsur-17
Plasmid#188859PurposeAvs inhibitor Lidtsur-17DepositorInsertLidtsur-17
UseTagsExpressionBacterialMutationPromoterJ23105Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lidtsur-6
Plasmid#188861PurposeAvs inhibitor Lidtsur-6DepositorInsertLidtsur-6
UseTagsExpressionBacterialMutationPromoterJ23105Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPtac
Plasmid#166937PurposePtac single-level gene expression controller (SLC)DepositorInsertGenetic circuit expressing GFP- PTAC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC
Plasmid#166941PurposeDual control based multi-level gene expression controller (MLC)DepositorInsertCircuit expressing GFP- Dual Control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTACAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH25_pBad_AraC_Para-sfGFP.0TAG
Plasmid#163720PurposeArabinose-inducible expression of green fluorescent protein encoding zero amber codonsDepositorInsertaraC gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB172a
Plasmid#156324PurposeExpresses heterologous rRNADepositorInsertrRNA operon, orthogonal-antiRBS
UseSynthetic BiologyTagsExpressionMutationWT antiRBS engineered to orthogonal antiRBSPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH23
Plasmid#107262Purposecontains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.DepositorInsertcurT (slr0483 )
UseSynthetic BiologyTagsmTurquoise2ExpressionBacterialMutationPromoternative CurT promoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP972
Plasmid#98806PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV T17S, N68D, E107D, D127A, F132L, S135F, F162S, K229E
UseTagsExpressionBacterialMutationT17S, N68D, E107D, D127A, F132L, S135F, F162S, K2…PromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p64-101
Plasmid#25911DepositorInsertIg germline gamma-1 switch region alpha
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRR5
Plasmid#225095Purposeparental riboswitch reporter vector for regulation of the gfpUV fluorescent proteinDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromotersyntheticAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only