We narrowed to 594 results for: pFAST

1. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

   Plasmid

   #187721

   Purpose

   Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 349-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-348

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1-His6-E3(1)-CUbo-TwinStrep

   Plasmid

   #212712

   Purpose

   Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Tags

   6xHis and CUbo-TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac HTB MBP-SARM1

   Plasmid

   #195763

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Use

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-Clover4-LUMABS-S1 RBD

   Plasmid

   #183491

   Purpose

   To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-S1 RBD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFASTbac-HisGST-PTPIP51(36-470)

   Plasmid

   #170531

   Purpose

   Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
   

   Use

   Tags

   GST tag and His tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-ATG101-ATG13 HORMA

   Plasmid

   #99332

   Purpose

   Mammalian expression of one subcomplex from the Human ULK1 complex

   Depositor

   James Hurley

   Article

   Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

   Inserts

   ATG101 (ATG101 Human)
   ATG13 (ATG13 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   p10 and pH

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac(His10-Lckg2a,Y394F)

   Plasmid

   #177893

   Purpose

   Baculo expression of His10 tag fused to human LckG2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Use

   Tags

   10xHis-TEV

   Expression

   Insect

   Mutation

   G2A,Y394F

   Promoter

   Polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac Dual α1B/β2B Tubulin

   Plasmid

   #170553

   Purpose

   Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

   Depositor

   Tarun Kapoor

   Article

   Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

   Inserts

   TUBA1B (TUBA1B Human)
   TUBB2B (TUBB2B Human)
   

   Use

   Tags

   AP linker, GS linker (GGSGG), His10 tag, L21 enha…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Use

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFAST-BAC BAF155/SMARCC1-His

    Plasmid

    #177863

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCC1 (SMARCC1 Human)
    

    Use

    Tags

    His and His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac-M13-6His-KIF1C-GFP

    Plasmid

    #130975

    Purpose

    Expression of 6His-KIF1C-GFP in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Use

    Tags

    6His and GFP

    Expression

    Insect

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual LIC cloning vector (5A)

    Plasmid

    #30121

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFAST-BAC BAF57/SMARCE1-His

    Plasmid

    #177861

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCE1 (SMARCE1 Human)
    

    Use

    Tags

    His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-Dual- GST-TEV-TBK1

    Plasmid

    #208875

    Purpose

    For expression in insect cells of TBK1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac (GST-preSci site-CD45)

    Plasmid

    #177889

    Purpose

    Baculo expression of GST tag fused to human CD45 ICD

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    CD45 (aa 598-1304 only) (PTPRC Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site

    Expression

    Insect

    Mutation

    L652P

    Promoter

    Polyhedrin

    Available since

    Aug. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid

    #64064

    Purpose

    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

    Depositor

    Andrew Carter

    Article

    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

    Insert

    Cytoplasmic dynein-2 (DYNC2H1 Human)
    

    Use

    Tags

    GFP, His tag, and ZZ tag

    Expression

    Insect

    Mutation

    Deleted amino acids 1-1090, extra C-terminal vali…

    Promoter

    Polyhedrin

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1-OSF-TRIM5alpha(human)(R332P)

    Plasmid

    #79037

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human R332P) (TRIM5 Human)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Changed Arg 332 to Pro

    Promoter

    polyhedrin

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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