We narrowed to 597 results for: pFAST

1. pFastBac1-ScVip1-186-1107

   Plasmid

   #238316

   Purpose

   ScVip1-186-1107 protein expression in insect cells

   Depositor

   Michael Hothorn

   Article

   Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

   Insert

   VIP1 (VIP1 Budding Yeast)
   

   Tags

   His10-Tag, Twin-Strep-tag, TEV

   Expression

   Insect

   Mutation

   ScVip1-186-1107

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac SOX 318-320A

   Plasmid

   #192060

   Purpose

   Expression vector for SOX (ORF37) 318-320A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37)
   

   Expression

   Insect

   Mutation

   318-320A

   Promoter

   Polyhedrin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

   Plasmid

   #187721

   Purpose

   Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 349-975 (NEDD4L Human)
   

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-348

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-His6-E3(1)-CUbo-TwinStrep

   Plasmid

   #212712

   Purpose

   Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Tags

   6xHis and CUbo-TwinStrep

   Expression

   Bacterial

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac HTB MBP-SARM1

   Plasmid

   #195763

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-Clover4-LUMABS-S1 RBD

   Plasmid

   #183491

   Purpose

   To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-S1 RBD
   

   Expression

   Insect

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFASTbac-HisGST-PTPIP51(36-470)

   Plasmid

   #170531

   Purpose

   Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 )
   

   Tags

   GST tag and His tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac(His10-Lckg2a,Y394F)

   Plasmid

   #177893

   Purpose

   Baculo expression of His10 tag fused to human LckG2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Tags

   10xHis-TEV

   Expression

   Insect

   Mutation

   G2A,Y394F

   Promoter

   Polyhedrin

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-M13-6His-KIF1C-GFP

    Plasmid

    #130975

    Purpose

    Expression of 6His-KIF1C-GFP in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    KIF1C-GFP (KIF1C Human)
    

    Tags

    6His and GFP

    Expression

    Insect

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid

    #64064

    Purpose

    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

    Depositor

    Andrew Carter

    Article

    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

    Insert

    Cytoplasmic dynein-2 (DYNC2H1 Human)
    

    Tags

    GFP, His tag, and ZZ tag

    Expression

    Insect

    Mutation

    Deleted amino acids 1-1090, extra C-terminal vali…

    Promoter

    Polyhedrin

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Insect

    Promoter

    Polyhedron

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual LIC cloning vector (5A)

    Plasmid

    #30121

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFAST-BAC BAF155/SMARCC1-His

    Plasmid

    #177863

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCC1 (SMARCC1 Human)
    

    Tags

    His and His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAST-BAC BAF57/SMARCE1-His

    Plasmid

    #177861

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCE1 (SMARCE1 Human)
    

    Tags

    His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-Dual- GST-TEV-TBK1

    Plasmid

    #208875

    Purpose

    For expression in insect cells of TBK1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST-TEV

    Expression

    Insect

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac (GST-preSci site-CD45)

    Plasmid

    #177889

    Purpose

    Baculo expression of GST tag fused to human CD45 ICD

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    CD45 (aa 598-1304 only) (PTPRC Human)
    

    Tags

    TEV-GST-PreScission cut site

    Expression

    Insect

    Mutation

    L652P

    Promoter

    Polyhedrin

    Available Since

    Aug. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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