We narrowed to 13,294 results for: SHI

1. pT7-gRNA:Asip

   Plasmid

   #60968

   Purpose

   Expression vector of rat Asip guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   Agouti signaling protein gRNA (Asip Rat)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pT7-gRNA:kit-1

   Plasmid

   #60969

   Purpose

   Expression vector of rat Kit-1 guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pT7-gRNA:kit-2-1

   Plasmid

   #60970

   Purpose

   Expression vector of rat Kit-2-1 guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pT7-gRNA:kit-2-2

   Plasmid

   #60971

   Purpose

   Expression vector of rat Kit-2-2 guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMRXIP-GFP-Spo20(PABD)

   Plasmid

   #221758

   Purpose

   Stable expression of GFP-Spo20(PABD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   Spo20 (SOS1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   tandem Spo20(PABD) (amino acids 422-551)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRXIB-mRuby3-LC3

   Plasmid

   #221738

   Purpose

   Stable expression of mRuby3-LC3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C2-MVP

   Plasmid

   #204543

   Purpose

   Expresses EGFP-tagged MVP in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IP-TMEM192-mKeima

   Plasmid

   #215358

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRX-IB-TMEM192-mKeima

   Plasmid

   #215359

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HR180-LGR5-GFP

    Plasmid

    #129092

    Purpose

    KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    EGFP and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Target-ACEmax

    Plasmid

    #139105

    Purpose

    Mammalian Target-ACEmax expression vector (pSI-625)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

    Plasmid

    #165430

    Purpose

    Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    FHS-paCaMKII(SD)
    

    Use

    AAV

    Tags

    FHS(Flag, HisX6, Strep) tag

    Expression

    Mammalian

    Mutation

    C450A, L514K, G528A, L531E, N538E: Light-insensit…

    Promoter

    CaMKII0.4

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HR180-LGR5-CreER

    Plasmid

    #129093

    Purpose

    KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    CreERT2 and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_023d-sgCiCh2-2

    Plasmid

    #202444

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Chromosome 2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

    Plasmid

    #186987

    Purpose

    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-s1006-gggs-d122, Gluc crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    s1006-gggs-d1221 deletion

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

    Plasmid

    #186993

    Purpose

    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-s1006-gggs-d122, non-targeting crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    s1006-gggs-d1221 deletion

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid

    #165435

    Purpose

    Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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