We narrowed to 25,261 results for: lars

1. pGEX4T3-GST-PolB(K206A/K244A/T304I)

   Plasmid

   #177134

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

   Plasmid

   #126651

   Purpose

   B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   Lrig1 (Lrig1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   C-terminal fusion of T2A-sfGFP-iCre-ERT2

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-beta2(616-951)Double

   Plasmid

   #100746

   Purpose

   Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Long isoform with deletion of clathrin binding mo…

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-beta2(616-951)Y815A

   Plasmid

   #100744

   Purpose

   Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Long isoform with Y815A mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-beta2(616-951)∆CBM

   Plasmid

   #100745

   Purpose

   Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   beta2 adaptin (AP2B1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Long isoform with deletion of clathrin binding mo…

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5b HA Irx5 deltaHD

   Plasmid

   #24997

   Depositor

   Benoit Bruneau

   Article

   Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

   Insert

   Irx5 (Irx5 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletes entire HD (aa 112-177)

   Promoter

   Available since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pU6-Osp.gRNA-MS2in

   Plasmid

   #229772

   Purpose

   Contains the U6 promoter and an optimized gRNA backbone inserted with two copies of the MS2 stem loop.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

   Insert

   gRNA cassette with two MS2 stem loops
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3-EF1A-mTert

   Plasmid

   #162555

   Purpose

   Overexpression of Tert

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   Overexpression of Tert (Tert Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF1A

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-PalmGRET

   Plasmid

   #158221

   Purpose

   BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   PalmGFP-Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mini-YFP-hDF-C2F-TM

    Plasmid

    #72600

    Purpose

    mini human dysferlin (from domain F to transmembrane ) tagged with YFP

    Depositor

    Renzhi Han

    Article

    Cheng et al Nat Med. 2014 Oct;20(10):1187-92. doi: 1

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    from domain F to transmembrane domain

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLV-SspB-HaloTag-p63DH

    Plasmid

    #176129

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgCD71-2

    Plasmid

    #46918

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCD71 -2 (TFRC Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Expression

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid

    #233371

    Purpose

    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Inserts

    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    

    Use

    Lentiviral

    Tags

    FLAG, NES, and mEGFP

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. IL2R/hE-cadherin-cytotail

    Plasmid

    #45773

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Interleukin-2 receptor α subunit extracellular an…

    Promoter

    CMV

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid

    #178057

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-FLAG
    

    Use

    AAV

    Tags

    FLAG-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SSpB-HaloTag-p63DH

    Plasmid

    #176116

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SSpB-mCherry-p63DH

    Plasmid

    #176112

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SSpB-iRFP-p63DH

    Plasmid

    #176120

    Purpose

    Heterodimerization with iLID, RhoA activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    RHOGEF p63 (ARHGEF25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid

    #206259

    Purpose

    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    GTS mTagBFP
    

    Use

    Multimate/gateway entr 4

    Tags

    BFGT1 (Golgi localisation peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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