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pAID1.2-EF1a-CGFP-mAID

Plasmid

#140608

Purpose

Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pREDIT_MCP-RecT

Plasmid

#164803

Purpose

mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Insert

RecT

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-mU6-Luc2GO-PGK-Neo

Plasmid

#136905

Purpose

Lentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

Luc2CyGo

Use

Lentiviral

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT

Plasmid

#112705

Purpose

Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJEC726

Plasmid

#190814

Purpose

CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

Depositor

James Chappell

Article

Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

S. pyogenes dCas9 codon optimized for Streptomyce…

Promoter

SP30

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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rs214-minor

Plasmid

#113380

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2149943 minor allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NmScarlet-mAID

Plasmid

#140617

Purpose

Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0-G-Gal4-VPR

Plasmid

#229187

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Gal4-VPR

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-mCherry

Plasmid

#112738

Purpose

Express 2(KW)2-mCherry in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-mCherry

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMS17

Plasmid

#127088

Purpose

Integrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.

Depositor

Maggie Smith

Article

Rodriguez-Garcia et al Nucleic Acids Res. 2005 May 25;33(9):e87

Inserts

tcp830
TetRiS
terminator mmrt
Terminator fdt

Use

Streptomyce integration vector derived from phic3…

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pREDIT_Cas9-MS2-BB_BbsI

Plasmid

#164802

Purpose

REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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BFP Homology Donor

Plasmid

#149372

Purpose

Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Insert

CDDR BFP Homology Donor

Expression

Mammalian

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-CmCherry-mAID

Plasmid

#140604

Purpose

Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHiFiNG-P2A-Puro

Plasmid

#136903

Purpose

Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHiFiNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

Promoter

EF1s

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pB1H2w5-Prd

Plasmid

#18039

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

Paired (prd Fly)

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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