We narrowed to 160,279 results for: Gene

1. pOpen-T5gene122

   Plasmid

   #165544

   Purpose

   Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T5 DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOpen-T4gene30

   Plasmid

   #165554

   Purpose

   Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T4-DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pOpen-T7gene5

   Plasmid

   #165534

   Purpose

   DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T7 DNA Polymerase (unmodified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pOpen-T4gene43

   Plasmid

   #165524

   Purpose

   Polymerase that replicates viral genomic DNA.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T4 DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOpen-T5gene12/13

   Plasmid

   #165559

   Purpose

   Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T5 Exonuclease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pOpen-T7gene1

   Plasmid

   #165505

   Purpose

   Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T7 RNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. WT lamin A minigene

   Plasmid

   #20291

   Depositor

   Tom Misteli

   Article

   Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

   Insert

   lamin A (LMNA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pOpen-T3gene1

   Plasmid

   #165576

   Purpose

   DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T3 RNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGENE:EFF-1::mCherry::V5

   Plasmid

   #133020

   Purpose

   EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

   Insert

   EFF-1::mCherry::V5
   

   Use

   Tags

   V5; mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS_KS_attB2_SA(0)_T2AGeneSwitch

    Plasmid

    #125214

    Purpose

    Artificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GeneSwitch
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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