We narrowed to 5,570 results for: donor

1. AICSDP-078:G3BP1-mEGFP

   Plasmid

   #193920

   Purpose

   Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

   Depositor

   Allen Institute for Cell Science

   Insert

   G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. LT2_14_GlyOx

   Plasmid

   #177711

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   pTRX2_5xUAS-ymYPET-tCYC1
   

   Use

   Used as donor dna in genome integration after lin…

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-WIPI2 HRD

   Plasmid

   #207544

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human WIPI2 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-UGCG-tdTomato

   Plasmid

   #225879

   Purpose

   Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

   Depositor

   Yohei Sato

   Article

   Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mMaroon-PAK-mMaroon

   Plasmid

   #84361

   Purpose

   FRET acceptor for Cdc42 donor, flanked with the far-red FP mMaroon

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mMaroon-PAK-mMaroon
   

   Tags

   mMaroon flanking PAK domain on N and C terminus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRE57-Ter

   Plasmid

   #170635

   Purpose

   DNA donor for transposition

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   Mini-Tn6677, Ter cassette
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOC1 Halo-Actb KI

   Plasmid

   #183426

   Purpose

   CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo donor
   

   Use

   CRISPR

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mMaroon-RTK-mMaroon

   Plasmid

   #84359

   Purpose

   FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mMaroon-Rtk--mMaroon
   

   Tags

   mMaroon flanking Rtk domain on N and C terminus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYZ172

   Plasmid

   #98409

   Purpose

   Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Inserts

   upstream homologous region to delete lys9 in genome by recombination
   downstream homologous region to delete lys9 in genome by recombination
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AICSDP-83:EZH2-mEGFP

    Plasmid

    #164499

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2

    Depositor

    Allen Institute for Cell Science

    Insert

    EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pOC2 Tubb3-GFP KI

    Plasmid

    #183432

    Purpose

    CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. KHBD00222

    Plasmid

    #34370

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1832 (CG1832 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pENTR macroH2A1.2-C

    Plasmid

    #30512

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Gateway - donor vector

    Mutation

    NO STOP

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1075

    Plasmid

    #111093

    Purpose

    Expresses eGFP donor in pUC19

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGM9121_Kma-Inulinase SP

    Plasmid

    #153492

    Purpose

    Kluyveromyces marxianus Inulinase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Inulinase SP
    

    Use

    Synthetic Biology

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOC1 Gria1-GFP-FRB KI

    Plasmid

    #183428

    Purpose

    CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP-FRB donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOC2 Dlg4-Halo-FKBP KI

    Plasmid

    #183435

    Purpose

    CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo-FKBP donor
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOC3 smFP-HA-Cacna1e KI

    Plasmid

    #183436

    Purpose

    CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFETCh_ONECUT2

    Plasmid

    #135738

    Purpose

    Donor vector for 3' FLAG tag of human ONECUT2

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ONECUT2 Homology arms (ONECUT2 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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