Addgene

We narrowed to 2,757 results for: fas

Showing: 2001 - 2020 of 2757 results

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pTER E2F3shRNA human

Plasmid

#66885

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F transcription factor 3 (E2F3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Tet

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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MO70 - Orai1 EC-Flag

Plasmid

#79590

Purpose

Transient expression and retroviral Expression

Depositor

Yousang Gwack

Article

Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

FLAG (inserted between TM3 and TM4 of the protein…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
pET-45b-NFYC-IDR-mEGFP

Plasmid

#185015

Purpose

Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

NFYC IDR (Nfyc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1

Plasmid

#111859

Purpose

Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Use

Tags

His-tag, TEV site, and ZZ

Expression

Insect

Mutation

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGY416

Plasmid

#166660

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

GAL1

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-hFOXH1GR

Plasmid

#59311

Purpose

Forced expression of human FOXH1GR

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

FOXH1 (FOXH1 Human)

Use

Retroviral

Tags

cGR - hormone binding domain of glucocorticoid re…

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1a-CRY2PHR-SID4X

Plasmid

#63663

Purpose

AAV expression of CRYS2PHR-SID4X

Depositor

Dong Kong

Article

Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

Insert

SID4X-CYR2PHR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA3

Plasmid

#59308

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiPOLR2D

Plasmid

#229022

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

POLR2D gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiPELO #2

Plasmid

#229020

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgCh2

Plasmid

#229023

Purpose

Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_174_neoR

Plasmid

#228935

Purpose

Lentiviral expression of EnAsCas12a

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgFOCAD #2

Plasmid

#229024

Purpose

Expression of a EnAsCas12a guide targeting FOCAD

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

FOCAD gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_051d_sgCiChr2-2

Plasmid

#228940

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSems StayGold-2xFYVE

Plasmid

#222948

Purpose

Expression of HRS 2xFYVE tagged with StayGold in mammalian cells

Depositor

Jacob Piehler

Article

Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Use

Tags

StayGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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