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pBS mPrx1 (CT#640)

Plasmid

#13961

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 promoter (Prrx1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRS416-PTEF1-MCP-no_intron

Plasmid

#127597

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

1xFLAG-MCP expression cassette

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJOG130

Plasmid

#105336

Purpose

Gateway entry clone generation

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG131

Plasmid

#105337

Purpose

Gateway entry clone generation

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA2

Plasmid

#30310

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α2 (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9wt

Plasmid

#20368

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pET30b-7Y-SrtA

Plasmid

#105601

Purpose

expresses 7Y sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7Y mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMyCA-mCherry

Plasmid

#84272

Purpose

mCherry fluorescence protein expression using M. smegmatis

Depositor

Young-Hwa Song

Article

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Insert

mCherry

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pESC-URA-Ub-G76A-Rnq1-GFP

Plasmid

#21052

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP and ubiquitin

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG417

Plasmid

#105341

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG418

Plasmid

#105342

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRS426-ACT_stable

Plasmid

#127609

Purpose

high-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Stable ACT1 intron (Mut)

Expression

Yeast

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pOG2003

Plasmid

#49516

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Tags

FokI-RR, HA, and SV40NLS

Expression

Worm

Promoter

hsp-16.2

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBN8

Plasmid

#86711

Purpose

Cloning plasmid for MosSCI transgenesis in C. elegans

Depositor

Peter Askjaer

Article

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Type

Empty backbone

Expression

Worm

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pOG2002

Plasmid

#49514

Purpose

TALEN backbone for expression of TALE(deltaRDV)-FokI AS in C. elegans

Depositor

Guangshuo Ou

Article

Cheng et al Nat Biotechnol. 2013 Oct;31(10):934-7. d

Type

Empty backbone

Tags

3xFLAG, FokI-AS, and SV40 NLS

Expression

Worm

Promoter

hsp-16.2

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pOX-twk16

Plasmid

#16444

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

C.elegans Tandem pore domain K+ channel twk-16 (twk-16 Nematode)

Use

Xenopus oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
xfz8N/pCS2+

Plasmid

#16322

Depositor

Peter Klein

Article

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-8 (fzd8.S Frog)

Use

Vertebrate expression

Mutation

Extracellular ligand binding domain of frizzled-8…

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pRS426-ECM_unstable

Plasmid

#127607

Purpose

high-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ECM33 intron (Mut)

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pVKG1-BSD

Plasmid

#108680

Purpose

Blasticidin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

SV40pro-BSD-polyA

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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