We narrowed to 9,957 results for: pET

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pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Publication

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Expression

Bacterial and Mammalian

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET

Plasmid

#118455

Purpose

Overexpression of ymYPET

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Expression

Plant

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Publication

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Publication

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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USPL1cat-pETM30

Plasmid

#85768

Purpose

bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

Depositor

Frauke Melchior

Publication

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

GST and His

Expression

Bacterial

Mutation

catalytic domain (aa 212-514)

Promoter

T7-lac

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet-T7-Ct-WspR

Plasmid

#79166

Purpose

Dual expression of Ct biosensor and WspR WT under individual T7 promoters

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

WspR (wspR P. areuginosa)
T7-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b/bPanK

Plasmid

#73619

Purpose

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

Depositor

Suzanne Jackowski, T. J. Kappock

Publication

Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Insert

pantothenate kinase (coaA Escherichia coli)

Tags

His6

Expression

Bacterial

Mutation

relative to MG1655 sequence, changes glutamate-5 …

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pETM6-G6-vioABECD

Plasmid

#66536

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET15b-Opt12

Plasmid

#61697

Purpose

Expresses PTDH mutant Opt12

Depositor

Huimin Zhao

Publication

McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

Insert

Opt12

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only
pETCON-HtsptLB12

Plasmid

#45120

Depositor

David Baker

Publication

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

HtsptLB12

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Publication

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL016A

Plasmid

#42135

Depositor

Sheref Mansy

Publication

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mYPet-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTs

Plasmid

#31173

Depositor

Linda Spremulli

Publication

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Ts

Tags

His

Expression

Bacterial

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
Ubp8/PET32a

Plasmid

#26965

Depositor

Cynthia Wolberger

Publication

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Ubp8 (UBP8 Budding Yeast)

Tags

His6 and Trx

Expression

Bacterial

Mutation

TEV site is added t the 5' end and 4 alanine…

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28a-EgC*

Plasmid

#20378

Depositor

Andrew Lee

Publication

Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

Insert

Eglin C wt*

Expression

Bacterial

Mutation

F10W

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only

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pET32b-FLJ23445

Plasmid

#15434

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445

Tags

His and Trx

Expression

Bacterial

Mutation

N/A

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET23a-LIR-1 D1-D2

Plasmid

#12103

Depositor

Pamela Bjorkman

Publication

Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

Insert

Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)

Expression

Bacterial

Mutation

Amino acids 1−198*

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only