-
-
-
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK057
Plasmid#75036PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
UseTagsExpressionBacterialMutationchanges residues 2-7 to His (SSRNNP>HHHHHH)PromoterT7 and n/aAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK350
Plasmid#71560PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)DepositorInsertalanine racemase
UseTagsHis6ExpressionBacterialMutationmutation changes alanine-365 to aspartatePromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJEC779
Plasmid#234348PurposeModular RENDR, GFP output, targets synthetic targetDepositorInsertModular RENDR, GFP output
UseSynthetic BiologyTagsExpressionMutationPromoterPJ23119Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJK606
Plasmid#72529PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsHis6ExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pJK290
Plasmid#71554PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges lysine-39 to alanine and tyrosine-260 to …PromoterT7Available sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only
