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We narrowed to 2,486 results for: EFS

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Showing: 2021 - 2040 of 2486 results

  1. LSB-hsa-let-7g-3p

    Plasmid
    #103157
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-let-7g-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-let-7g-3p target (MIRLET7G Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103157-plasmid-map-sequence-id-213247

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  2. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep
    #98747-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  3. pAAV-FLEX-EGFPL10a (AAV Retrograde)

    Viral Prep
    #98747-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  6. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Available Since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

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  11. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  15. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Article
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    170069-plasmid-map-sequence-id-333408

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  19. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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Showing: 2021 - 2040 of 2486 results