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EFS-Vector
Plasmid#109395PurposeAAV-CRISPR knockout plasmid for direct in vivo genome editingDepositorTypeEmpty backboneUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterEFs-NSAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9
Plasmid#210703PurposeThis plasmid express EFS promoter driven human codon optimized SpdCas9DepositorInsertS. Pyogenes dCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A/H840APromoterEF1a core promoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
EFS-GFP
Plasmid#110834Purposepositive control for GFP expressionDepositorInsertelongation factor 1alpha binding sequence (EFS) at GFP promoter region
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOPO EFS
Plasmid#68451PurposeTOPO construct expressing EFS for generation of GMAP-compatible promoterDepositorInsertElongation Factor 1α Promoter
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLEL_6 [EF]
Plasmid#199031PurposeOdd assembly end-linkerDepositorInsertEF linker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF
Plasmid#177267PurposeCRISPR vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef
Plasmid#127721PurposeLevel 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEFS and hU6Available sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.tRFP
Plasmid#57819PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.PAC
Plasmid#57828PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.dTomato
Plasmid#89395PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.E2Crimson
Plasmid#100894PurposeLentiviral gRNA deliveryDepositorInsertE2Crimson
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
HeFSpCas9
Plasmid#92355PurposeExpression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, R1060APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9
Plasmid#126766PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.DepositorInsertB-HeFSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…PromoterCBhAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.RFP657
Plasmid#69147PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEFS 4r-3r
Plasmid#20100DepositorInsertIntron (p1)
UseSynthetic Biology; Plant gateway vectorTagsExpressionMutationPromoterAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEFS 3-2
Plasmid#20107DepositorInsertIntron (p2)
UseSynthetic Biology; Plant gateway vectorTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.pre
Plasmid#169276PurposeConstitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EFS-1/1
Plasmid#91456PurposeProtein expression and purification of human SH3 domain construct EFS-1/1DepositorInsertEFS-1/1 (EFS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only
