We narrowed to 2,775 results for: PAM
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pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
C3 luc (T1 del)
Plasmid#11358DepositorInsertC3 promoter (C3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-E2419K
Plasmid#19994DepositorInsertmammalian target of rapamycin-E2419K (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Glutamine 2419 to LysinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.2-GFP
Plasmid#72908PurposeGFP-tagged mSin1 (isoform 2)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-L1460P
Plasmid#19995DepositorInsertmammalian target of rapamycin-L1460P (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Leucine 1460 to ProlinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 S6K2 E388 D3E
Plasmid#17731DepositorInsertS6K2 (RPS6KB2 Human)
UseTagsHAExpressionMammalianMutationT388E, D3E(S410D, S417D, S423D): rapamycin-resist…PromoterCMVAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.5-GFP
Plasmid#72909PurposeGFP-tagged mSin1 (isoform 5)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
gCOTS-pyl
Plasmid#92048PurposeThis is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.DepositorInsertsPyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseCyanobacteria s. elongatus genome recombination i…TagsExpressionMutationPromoterLeuP (native S. elongatus promoter) and PrcbLAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-CgE
Plasmid#13762DepositorInsertHSV-1 glycoprotein E
UseTagsHisExpressionInsectMutationC-terminal region of the HSV-1 gE (KOS strain ect…PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSP3-bio
Plasmid#47731PurposeExpresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP3
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
A2M-bio-His
Plasmid#53423PurposeExpresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertA2M (A2M Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(TIAM1)
Plasmid#85156Purposeacute activation of Rac through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
alpha1Ddelta-G12-FRB
Plasmid#87454Purposerapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCsDepositorInserta1D short variants (Cacna1d Rat)
UseTagsFRBExpressionMammalianMutationdelete KYPAKNTTIALQMLERMLPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-664
Plasmid#92252PurposePlasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterEF1AAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-658
Plasmid#92257PurposePlasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motifDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsNHAExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hADM-RFP
Plasmid#22922DepositorInserthuman adrenomedullin promoter driving DsRedExpress (ADM Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only
