We narrowed to 17,224 results for: emb

1. pCAG-3xGFP11-mem-3xPax7gRNA

   Plasmid

   #224571

   Purpose

   Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   3xGFP11
   

   Use

   CRISPR

   Tags

   Membrane Localization Signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL NarL YdfI Flag

   Plasmid

   #225650

   Purpose

   Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarL-YdfI (narL Synthetic)
   

   Use

   Cell free expression

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   NarL with native DNA-binding domain replaced with…

   Promoter

   T7

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

   Plasmid

   #207025

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A I375A D384A Y387A

   Promoter

   pTEF1

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCUP1-GFP-Osh1-406

   Plasmid

   #207007

   Purpose

   Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   OSH1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

   Plasmid

   #207050

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   P85L P86L

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

   Plasmid

   #207051

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   T56A R65A T87A

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

   Plasmid

   #207052

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   R65A P85A P86A

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

   Plasmid

   #207053

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   R65A P85L P86L

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPIP1;4-NLS-3xmCitrine

   Plasmid

   #192524

   Purpose

   Fluorescent reporter for plant responses to bacterial infection

   Depositor

   Gitta Coaker

   Article

   Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

   Insert

   PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
   

   Use

   Tags

   3xmCitrine and NLS

   Expression

   Plant

   Mutation

   Promoter

   PIP1;4

   Available since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pS238D1::tse5-CT

    Plasmid

    #192958

    Purpose

    For expression of Tse5-CT (1169-1317) in P. putida for biological function studies

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1317)

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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