Addgene

We narrowed to 5,747 results for: cki

Showing: 2041 - 2060 of 5747 results

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psgRNA2.02-PP7-ccdB

Plasmid

#99912

Purpose

Basic cloning vector for the expression of PP7 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pNICE HA-mSYD1B

Plasmid

#59362

Purpose

Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)

Depositor

Peter Scheiffele

Article

Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

Insert

mSYD1B (Syde2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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psgRNA2.02-MS2-ccdB

Plasmid

#99911

Purpose

Basic cloning vector for the expression of MS2 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF05

Plasmid

#52558

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF05

Use

Tags

Expression

Insect

Mutation

changed from GCaMP3: D397N/G398A/N399D

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 L232A

Plasmid

#8976

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

L232A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pR540

Plasmid

#207885

Purpose

template for 2 gRNA amplification

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

scaffold-pU6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCS021

Plasmid

#207917

Purpose

Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKE013

Plasmid

#207916

Purpose

Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCh_NFIA_iso1

Plasmid

#104039

Purpose

Donor vector for 3' FLAG tag of human NFIA_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 homology arms (NFIA Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_2

Plasmid

#104049

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_1

Plasmid

#104048

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK2 Y261A

Plasmid

#8977

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Y261A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pFETCh_NFIL3

Plasmid

#86287

Purpose

Donor vector for 3' FLAG tag of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIL3 homology arms (NFIL3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_2

Plasmid

#86349

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_1

Plasmid

#86348

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Expression

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-hNKD2

Plasmid

#174328

Purpose

encodes human NKD2 cDNA with a C-terminal GFP-fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

GFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

Plasmid

#171429

Purpose

Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C261

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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