We narrowed to 5,543 results for: pcDNA3.1+
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Plasmid#236419Purposetransient overexpression of EMSY in mammalian cellsDepositorInsertEMSY (2210018M11Rik Mouse)
ExpressionMammalianAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)
Plasmid#238007Purpose300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.DepositorUseRna donor to study rt-dsbrExpressionMammalianMutation3bp insertion signature (ATC) at Cas9 cut site in…PromoterCMVAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT
Plasmid#233064PurposeExpresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G
Plasmid#233063PurposeExpresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.DepositorInsertADGRL3 (Adgrl3 Mouse)
TagsALFA and HiBiTExpressionMammalianMutationThreonine 923 to GlycineAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Cas9-P2A-dTomato-PS9
Plasmid#231911PurposeFor packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signalDepositorInsertCas9-P2A-dTomato
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Cas9-P2A-dTomato-T20
Plasmid#231912PurposeFor packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signalDepositorInsertCas9-P2A-dTomato
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 T21R-Nb139-T2A-mCherry
Plasmid#225589PurposeMammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-Nb139-T2A-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA
Plasmid#228532PurposeA fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.DepositorInsertmyc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
TagsHA and mycExpressionMammalianPromoterCMVAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-GTSE1-S91, 262 ,724A
Plasmid#224737PurposeFor expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutationDepositorInsertGTSE1 (GTSE1 Human)
TagsHAExpressionMammalianMutationSerine 91, 262 ,724 to AlaninePromoterCMVAvailable SinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)
Plasmid#227619PurposeOverexpression of SLC25A46 lacking its N-terminal region in mammalian cell linesDepositorInsertSLC25A46 (SLC25A46 Human)
TagsP2A-mElectra1ExpressionMammalianMutationdeleted aa 2-83PromoterCMV, T7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA
Plasmid#222861PurposeThis plasmid codes for the OgeuIscB nickaseDepositorInsertOgeuIscB Nickase
UseCRISPRTags3X HA and Nucleoplasmin NLS and ITR2ExpressionMammalianMutationE193A for nickase mutationPromoterCMVAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT
Plasmid#215898PurposeExpression of TNRC6A-CIM1 domainDepositorAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT
Plasmid#215899PurposeExpression of TNRC6A-CIM2 domainDepositorAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(G12C, K96H)
Plasmid#224281PurposeCell transfection and expression of Rac1 (G12C, K96H)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)
Plasmid#224277PurposeCell transfection and expression of Rap1A (G12C, L96F)DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-R42A
Plasmid#221684PurposeMammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioIDDepositorAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Heavy chain-SpyTag003
Plasmid#216303PurposeExpresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-SpyTag003
Plasmid#216293PurposeExpresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink FabDepositorInsertTras heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Heavy chain-SpyTag003
Plasmid#216296PurposeExpresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S FabDepositorInsert39S Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT
Plasmid#218460PurposeMammalian expression plasmid for SARS-CoV-2 spike (1273 residues)DepositorInsertSARS-CoV-2 spike protein (1273 residues)-wild type (S )
ExpressionMammalianAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only