We narrowed to 10,060 results for: plasmids 101
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Plasmid#112667PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pT3-EF1aH
Plasmid#180149PurposeThis plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP)Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK187
Plasmid#156423PurposeInsect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein
UseTagssfGFP-HisExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(SKNCKS)-P2A-EGFP (RAS2951)
Plasmid#223101PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with SKNCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(SKNCKS)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(SKNCKS)=D1135S, S1136K, G1218N, E1219C, R1…PromoterCMV and T7Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Kv1.1 K+ channel scFv [K20/78]
Plasmid#190486PurposeMammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.DepositorInsertKv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid#60231PurposeExpresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
EGFP
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA-P2A and EGFP-KASHExpressionMammalianMutationPromoterU6 and hSynAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
Plasmid#60226PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Renilla luciferase
Cre recombinase
UseAAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…TagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112669PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHC042 (∆phaCAB)
Plasmid#226208PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.DepositorInsertHomology arms for phaCAB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2340_Tier3(SB)-iRFP_Puro
Plasmid#169638PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCHC039 (∆phcA)
Plasmid#226207PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.DepositorInsertHomology arms for PhcA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZac2.1 GfaABC1D rM3D mCherry SV40
Plasmid#92285PurposeAAV DREADD expressionDepositorInsertrM3D
UseAAVTagsmCherryExpressionMutationPromoterGfaABC1DAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K9R
Plasmid#84527PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK(43,61,69,117,240,246,527,582,651)RPromoternoneAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK193
Plasmid#156424PurposeInsect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (Q57H)
UseTagssfGFP-HisExpressionInsectMutationQ57HPromoterPolyhedrinAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pProvector_AAV2_586_PLGLAR
Plasmid#129024PurposeRecombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.DepositorInsertRep2/Cap2 with PLGLAR lock at 586
UseAAVTagsExpressionMutationinserted peptide motif (PLGLARGDDDDGPLGLAR) at po…PromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only