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pJL1_CSL3

Plasmid

#200173

Purpose

Amino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

Depositor

Michael Jewett

Publication

Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

Insert

CSL3

Tags

6x His tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1_SUL-I

Plasmid

#200172

Purpose

Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

Depositor

Michael Jewett

Publication

Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

Insert

SUL-I

Tags

6x His tag and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:HaloTag

Plasmid

#179785

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG::HaloTag

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-lssmOrange

Plasmid

#179795

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

lss mOrange

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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NK617

Plasmid

#109095

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Publication

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

S. cerevisiae CoxIV target sequence

Use

Choanoflagellate expression

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Phleo/V5

Plasmid

#1809

Depositor

Christian Tschudi

Publication

Shen et al Mol Biochem Parasitol 2001 Mar;113(1):17

Insert

Phleo/V5

Tags

V5

Expression

Bacterial

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only
WT MBP-pDosa1

Plasmid

#260650

Purpose

Bacterial expression of MBP fused to tRNA modifying enzyme TrmA (E358Q)

Depositor

Stan Fields

Publication

Ashok et al Proc Natl Acad Sci U S A. 2026 Jan 6;123

Insert

MBP

Tags

6xHis and TrmA (E358Q)

Expression

Bacterial

Promoter

T7

Available since

Oct. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-h2-3

Plasmid

#262788

Purpose

Constitutive expression of h2-3 fluorescent protein. Single-color control for tFucci(CA)5 and tFucci(SA)5

Depositor

Courtney Hodges

Publication

Cermakova et al Nucleic Acids Res. 2024 Jan 11;52(1):4-2

Insert

h2-3

Use

Piggybac

Expression

Mammalian

Promoter

CAG

Available since

Oct. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pX458-SLC35A1

Plasmid

#239952

Purpose

Encodes gRNA for human SLC35A1

Depositor

Jan Carette

Publication

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

SLC35A1

Use

CRISPR

Expression

Mammalian

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MFSD6

Plasmid

#239951

Purpose

Encodes gRNA for human MFSD6

Depositor

Jan Carette

Publication

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR

Expression

Mammalian

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR/HA-hBIG2

Plasmid

#226252

Purpose

Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

Depositor

Hye-Won Shin

Insert

BIG2 (ARFGEF2 Human)

Use

Gateway Cloning

Tags

HA

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTvGPI(VSG)-blast-mNG

Plasmid

#221050

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJTT002

Plasmid

#204493

Purpose

Reporter circuit

Depositor

Ron Weiss

Publication

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

TagBFP
EYFP
mKate2

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:mScarletI:3Ty

Plasmid

#201093

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3Ty:mScarletI:3Ty

Plasmid

#201092

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:mScarletI:3Ty

Plasmid

#201091

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-hygro-3Ty:tagRFPt:3Ty

Plasmid

#201088

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-g418-3Ty:tagRFPt:3Ty

Plasmid

#201087

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv6-blast-3Ty:tagRFPt:3Ty

Plasmid

#201086

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-BFP

Plasmid

#190259

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge