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pCDNA3-biotin-myc- mCE

Plasmid

#82475

Purpose

Expresses mRNA capping enzyme containing N terminal biotin and myc tag

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

Biotin (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 BICC FL

Plasmid

#112998

Purpose

For protein expression and purification of full-length Drosophila BicC

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length BicC (BicC Fly)

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx6

Plasmid

#108135

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]6:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]6:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx4

Plasmid

#108134

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]4:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]4:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5081

Plasmid

#85107

Purpose

Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP-KaiC

Tags

YFP

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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GST-RP2-C86Y,P95L

Plasmid

#86074

Purpose

A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Mutation

Changed cysteine-86 and proline-95 to tyrosine an…

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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GST-cystin-CTS

Plasmid

#86066

Purpose

A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Cystin (Cys1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 28-35

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pPD3cC

Plasmid

#86972

Purpose

Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

Promoter

T7 promoter

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Expression

Mammalian

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(WT)

Plasmid

#86576

Purpose

Expresses cleavable human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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