We narrowed to 10,060 results for: plasmids 101

1. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

   Plasmid

   #219426

   Purpose

   Cre-dependent expression of luciferase opsin fusion protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

   Insert

   mNeonGreen, ISN-IE, ChRmine
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Expression

   Mutation

   Promoter

   EF-1alpha

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

   Plasmid

   #203839

   Purpose

   Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dCre
   

   Use

   AAV

   Tags

   mScarlet-KASH-2A

   Expression

   Mutation

   Enzymatically inactive Cre

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPseq-BFP-backbone

   Plasmid

   #85707

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Pur-CAG-hM3Dq-mCherry

   Plasmid

   #80948

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM3Dq-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPseq-mCherry-backbone

   Plasmid

   #85708

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX nsp1 CoV2

   Plasmid

   #175512

   Purpose

   For protein expression of WT nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

   Plasmid

   #127864

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV-2-NSP3-c001

   Plasmid

   #173083

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E1084-E1192, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CoV-2-NSP3-c002

    Plasmid

    #173084

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDK197

    Plasmid

    #134189

    Purpose

    Retroviral plasmid expressing LAP-Clasp C terminus

    Depositor

    Iain Cheeseman

    Article

    Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

    Insert

    LAP-Clasp C terminus (CLASP1 Human)
    

    Use

    Retroviral

    Tags

    LAP (GFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221-rap1-K462-463-651R

    Plasmid

    #84564

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K462-463-651R

    Promoter

    none

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-rap1-K43-117-118R

    Plasmid

    #84565

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K43-117-118R

    Promoter

    none

    Available since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCHC022 (∆phcA)

    Plasmid

    #226200

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCHC004 (∆phaCAB)

    Plasmid

    #226198

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for phaCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGL4.11_Nued2_E5

    Plasmid

    #203834

    Purpose

    Luciferase reporter plasmid for Igf1 enhancer 5

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    E5 (Igf1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-nsp7

    Plasmid

    #201025

    Purpose

    Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet-ON

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVH666

    Plasmid

    #169610

    Purpose

    Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO7-PCMVmin / PPGK / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH664

    Plasmid

    #169608

    Purpose

    Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO7-PCMVmin / PPGK / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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