We narrowed to 9,753 results for: try

1. 460 p3E-2A-mCherrypA

   Plasmid

   #195970

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCherry
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-VP40

   Plasmid

   #74421

   Purpose

   Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

   Depositor

   Judith White

   Article

   Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

   Insert

   VP40
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. opto-Rac1

   Plasmid

   #135396

   Purpose

   Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

   Insert

   opto-Rac1
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAtetO_6xHis-Klentaq

   Plasmid

   #157862

   Purpose

   Plasmid for the aTc-inducible expression of the Klentaq

   Depositor

   Andrew Ellington

   Article

   Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

   Insert

   Klentaq
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   tet PA

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. H3K9me3Probe_FullLength

   Plasmid

   #186463

   Purpose

   Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

   Depositor

   Chongli Yuan

   Article

   Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

   Insert

   dimeric CDY1 (CDY1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 457 p3E-2A-EGFPpA

   Plasmid

   #195967

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-EGFP
   

   Use

   Other

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ES-FUCCI

   Plasmid

   #62451

   Purpose

   SMX_DEST vector with 3 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
   minPGK::Cherry-hCdt1(30-120)-bGHpA
   PGK::HygroR-bGpA
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. (+)-Limonene Synthase

    Plasmid

    #112230

    Purpose

    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

    Depositor

    Daniel Oprian

    Article

    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

    Insert

    (+)-Limonene Synthase
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-52

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE30-roGFP1-iR

    Plasmid

    #83314

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iR
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

    Promoter

    T5

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pEBFP2-Nuc

    Plasmid

    #14893

    Purpose

    Mammalian expression of EBFP2 blue fluorescent protein with NLS

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Insert

    pEBFP2-Nuc
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid

    #20938

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-GFP

    Plasmid

    #137763

    Purpose

    Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFGAMS-mCherry

    Plasmid

    #166024

    Purpose

    Expresses human PFAS-mCherry fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

    Insert

    PFAS-mCherry (PFAS Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet1_CoiA1_CoiB

    Plasmid

    #208761

    Purpose

    Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiA1
    CoiB
    

    Tags

    Hisx6 Tag and SUMO

    Expression

    Bacterial

    Mutation

    Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_PGK::ZeoR-bGHpA

    Plasmid

    #62442

    Purpose

    MXS_chaining vector with PGK::ZeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Zeocin with PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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