We narrowed to 23,428 results for: CRISPR

1. pCXLE-dCas9VPP300-T2A-GFP-shP53

   Plasmid

   #102896

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPP300-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

   Plasmid

   #85749

   Purpose

   Expresses anti-CRISPR protein from Type I-E and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type I-E anti-CRISPR
   mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_dCas9-2xAM_hIRF-1

   Plasmid

   #92221

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA targeting human IRF-1 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC43

   Plasmid

   #104817

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSC44

   Plasmid

   #104818

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC-OspCas12c-noncoding

   Plasmid

   #120878

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-OspCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ291

   Plasmid

   #129671

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   BthCas12b is rice codon optimized

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC45

   Plasmid

   #104819

   Purpose

   CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.04g057400
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC29

    Plasmid

    #104803

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Medtr4g094545
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-LmoCas6-p300

    Plasmid

    #126486

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas6-p300
    

    Use

    CRISPR

    Tags

    Flag and Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-CasE-p300-Flag

    Plasmid

    #106275

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE-p300
    

    Use

    CRISPR

    Tags

    E tag, NLS and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LC-E75

    Bacterial Strain

    #115925

    Purpose

    Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Bacterial Resistance

    None

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid

    #225986

    Purpose

    To make Gateway entry clone of CRISPR guide RNA under AtU6p

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRDA_887

    Plasmid

    #245329

    Purpose

    Cas12a CRISPRa cloning vector; contains NbALFA, p65

    Depositor

    John Doench, David Root

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSG804

    Plasmid

    #239534

    Purpose

    CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae

    Depositor

    John Dueber

    Insert

    sgRNA_YDR514C
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPSG779

    Plasmid

    #239532

    Purpose

    CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae

    Depositor

    John Dueber

    Insert

    sgRNA_YBR197C
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPSG803

    Plasmid

    #239533

    Purpose

    CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae

    Depositor

    John Dueber

    Insert

    sgRNA_YMR206W
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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