We narrowed to 151,920 results for: MAL

1. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Use

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCE-K2N

   Plasmid

   #154879

   Purpose

   Episomally expresses KLF2 and NANOG in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Insert

   KLF2-2A-NANOG (KLF2, NANOG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCE-KdGl

   Plasmid

   #154880

   Purpose

   Episomally expresses KDM4D and GLIS1 in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Insert

   KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBOBI-FLuc

   Plasmid

   #170674

   Purpose

   Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   luc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-FLuc

   Plasmid

   #170575

   Purpose

   Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   Luc
   

   Use

   Luciferase and Retroviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

   Plasmid

   #162877

   Purpose

   Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Insert consists of AA1-170

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

   Plasmid

   #162880

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-EGFP_Hu_p115-RhoGEF(WT)

   Plasmid

   #136337

   Purpose

   Mammalian expression of human p115-RhoGEF with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   ARHGEF1 (ARHGEF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

    Plasmid

    #132667

    Purpose

    Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-V5_Hu_p115-RhoGEF(WT)

    Plasmid

    #136335

    Purpose

    Mammalian expression of human p115-RhoGEF with N-terminal V5

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    ARHGEF1 (ARHGEF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDN-D2irTNG4kwh

    Plasmid

    #44722

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    htetR::NLS::EGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter;…

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-MBP [N128A/4R]

    Plasmid

    #206531

    Purpose

    Mammalian Expression Plasmid of anti-Maltose binding protein (MBP) (E. coli). Derived from hybridoma N128A/4.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Maltose binding protein (MBP) (Escherichia coli) recombinant Mouse monoclonal antibody (malE Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-EGFP-Hu_Plk1(WT)

    Plasmid

    #136323

    Purpose

    Retroviral expression of human Plk1-WT with N-terminal EGFP

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBacMam8_N_Flag_3C

    Plasmid

    #178818

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBacMam2_N_His8_eGFP_TEV

    Plasmid

    #178806

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBacMam3_N_His8_eGFP_3C

    Plasmid

    #178807

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    His8, eGFP, 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBacMam4_N_Flag_His8_eGFP_TEV

    Plasmid

    #178808

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, His8, eGFP, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBacMam7_N_Flag_TEV

    Plasmid

    #178817

    Purpose

    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

    Depositor

    Kazuhiro Abe

    Type

    Empty backbone

    Use

    Tags

    Flag, TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge