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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,254 results for: Xpa

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Showing: 2101 - 2120 of 2254 results

  5. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  6. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available Since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  7. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  9. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  10. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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  13. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85484-plasmid-map-sequence-id-169864

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  14. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available Since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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Showing: 2101 - 2120 of 2254 results