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pHH0103_SRC-1/1-IS2/2

Plasmid

#91251

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS2/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAO1_G-alpha

Plasmid

#110013

Purpose

Protein expression and purification of GNAO1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAO1_G-alpha (GNAO1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT2 NCmut-mApple

Plasmid

#180321

Purpose

mammalian expression of human SEPT2 NCmut fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT2 F20D, V27D

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-2/5

Plasmid

#91385

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-2/5 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-i5-msfGFP

Plasmid

#180332

Purpose

mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v7 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1-i5

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1-1/1-SV1/2

Plasmid

#91399

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV1/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR2_Hormone-recep

Plasmid

#109907

Purpose

Protein expression and purification of ESR2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR2_Hormone-recep (ESR2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DLG3_PDZ_3

Plasmid

#103948

Purpose

Protein expression and purification of NE-DLG PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-2/2

Plasmid

#91410

Purpose

Protein expression and purification of human SH3 domain construct GRB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-2/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRAP2-2/2

Plasmid

#91368

Purpose

Protein expression and purification of human SH3 domain construct GRAP2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP2-2/2 (GRAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8L2-1/1

Plasmid

#91526

Purpose

Protein expression and purification of human SH3 domain construct EPS8L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L2-1/1 (EPS8L2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_3

Plasmid

#103900

Purpose

Protein expression and purification of PTPN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CRK-2/2-SV2/2

Plasmid

#91343

Purpose

Protein expression and purification of human SH3 domain construct CRK-2/2-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRK-2/2-SV2/2 (CRK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MAGI1_PDZ_2

Plasmid

#103899

Purpose

Protein expression and purification of MAGI1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ASAP1-1/1

Plasmid

#91501

Purpose

Protein expression and purification of human SH3 domain construct ASAP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP1-1/1 (ASAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNMBP-6/6

Plasmid

#91489

Purpose

Protein expression and purification of human SH3 domain construct DNMBP-6/6

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DNMBP-6/6 (DNMBP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_KALRN-1/4

Plasmid

#91283

Purpose

Protein expression and purification of human SH3 domain construct KALRN-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

KALRN-1/4 (KALRN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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