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TMEM16F-I521K-peGFP-N1

Plasmid

#228588

Purpose

Expresses TMEM16F-I521K-peGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I521K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I611K-peGFP-N1

Plasmid

#228581

Purpose

Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I611K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-flex-Clover

Plasmid

#135177

Purpose

AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

Inserts

Clover
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of GFP

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p2R3a-3AT

Plasmid

#71273

Purpose

Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator

Use

Gateway

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pTH732-2µ-RLuc/staCFLuc

Plasmid

#40607

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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BPK2797

Plasmid

#72251

Purpose

Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335Q, and T1337R

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)

Plasmid

#190134

Purpose

expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells

Depositor

Joshua Knowles

Article

Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

Insert

eGFP-p2A-HA-GPR151(p.Arg95Ter)

Use

Lentiviral

Tags

HA, eGFP, and p2A

Expression

Mammalian

Mutation

p.Arg95Ter mutation

Promoter

CMV

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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cytosolic moPrP (3F4)

Plasmid

#1321

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

N-term truncation (to make gene cytosolic), vario…

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-ITR-EcYtR(NGS-6)-mCherry

Plasmid

#217363

Purpose

Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA mutant, "NGS-6"

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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