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pCellFree_G06_ZIKV NS5

Plasmid

#145358

Purpose

Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV E

Plasmid

#145361

Purpose

Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-STOP-IRES-Puro

Plasmid

#120558

Purpose

Expresses control HA-birA* in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

birA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

R118G

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-BA.4/5-AVI

Plasmid

#192114

Purpose

Expresses SARS-CoV-2 RBD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Insert

SARS-CoV-2-RBD-BA.4/5 (S )

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV C

Plasmid

#145359

Purpose

Cell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_N_ZIC3

Plasmid

#167809

Purpose

Expresses ZIC3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ZIC3 (ZIC3 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB6

Plasmid

#187763

Purpose

MAC-tagged gene expression of human EPHB6

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB6 (EPHB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_HEN1

Plasmid

#167708

Purpose

Expresses HEN1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

HEN1 (NHLH1 Human)

Use

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Use

Tags

V5; mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV PrM

Plasmid

#145360

Purpose

Cell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

March 23, 2024

Availability

Academic Institutions and Nonprofits only

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