We narrowed to 5,631 results for: chia

1. pSF280

   Plasmid

   #167675

   Purpose

   Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJM.474

   Plasmid

   #108526

   Purpose

   LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LuxR + PLuxB-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VanR: T49I A111V P179S

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAJM.771

   Plasmid

   #108534

   Purpose

   NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   NahRAM + PSalTTC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NahR: I25V P72H V118A R248C

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2CT-His-MBP-Lwa_Cas13a_WT

   Plasmid

   #91865

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LwaCas13a
   

   Use

   CRISPR

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMON-HsNMT1

   Plasmid

   #67475

   Purpose

   Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

   Depositor

   Richard Kahn

   Article

   Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

   Insert

   HsNMT1 (NMT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pKL003

   Plasmid

   #98919

   Purpose

   Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Insert

   PROPS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23118 - Strong Constitutive

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-mCherry-MinD-IRES-Puro

   Plasmid

   #213112

   Purpose

   Expression of mCherry-MinD in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. EcNusGFL

    Plasmid

    #113122

    Purpose

    Expresses full-length E. coli NusG

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    NusG (nusG Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SupD v2

    Plasmid

    #198598

    Purpose

    Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SupD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDR111_mannose_Bgal no NLS

    Plasmid

    #188399

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDUET-1_VNp-anti-6xHis mAb

    Plasmid

    #214952

    Purpose

    Bacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-6xHis mAb heavy chain
    VNp-anti-6xHis mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH847-gst-optsc

    Plasmid

    #96908

    Purpose

    Plasmid for yeast expression of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pScalps_Puro

    Plasmid

    #99636

    Purpose

    Empty backbone, 3rd generation lentiviral vector

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    SFFVp

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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