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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 57 results for: chia

Showing: 1 - 20 of 57 results

  2. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...devastating afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research Scientist ...mechanisms behind the disease are unclear. Dr. Di Schiavi plans on using C. elegans as a model to identify...disruption. As part of his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 ...behind SMN1 and its interaction partners, Dr. Di Schiavi will be able to better choose which genes and pathways...SMN1 and its partners in mouse cell lines. Dr. Di Schiavi hopes that these studies will accelerate the development...Website.  We are proud to provide support to Drs Di Schiavi and Nabavi and will continue to work to help those...

  3. CRISPR Library Amplification

    Type
    Protocol
    ...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...

  4. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying the abundance...time is time consuming and unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler's lab at...from each bin are then extracted and sequenced. Chiasson is currently using VAMP-seq to study vitamin K...scales and making personalized medicine a reality,” Chiasson explains. Elizabeth Unger: Developing a serotonin...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. ...2017). Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli -...environments (Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID. E. coli is often a...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...formerly known as Pichia pastoris). If your goal is protein isolation, then the MoClo Pichia Kit will get ...fungi, bacteria, protists, plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters...Ulrike, et al. “A Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology...Crossref, https://doi.org/10.1016/j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden...

  7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter...first described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification..., Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated...counter-selectable marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...vulgaris, Erwinia carotovora, E. chrysanthemi, Escherichia coli, Gluconacetobacter xylinus, Gluconobacter...E. herbicola, E. rubrifaciens, E. stewartii, Escherichia coli, Klebsiella spp., Legionella pneumophila...spp., Brucella spp., Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus, Paracoccous ...integron promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens. 3 Biotech...

  9. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated...enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015)...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed...

  12. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...

  13. Bacterial Expression Systems

    Type
    Collection
    ...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...pBAD Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl...Lactose/IPTG Escherichia coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas... Lactose/IPTG Escherichia coli Cheryl Arrowsmith 46886 pMSP3535 PnisA Nisin Escherichia coli , gram-positive...ε-caprolactam Escherichia coli , Streptomyces sp. Xuming Mao 46888 pMSP3545 PnisA Nisin Escherichia coli , gram-positive...pPrpB Propionate Escherichia coli Jay Keasling 17810 pPro33 pPrpB Propionate Escherichia coli Jay Keasling...sensing molecule) Escherichia coli Cynthia Collins 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize ...et al., 2017). They characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion...

  15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...levels in general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

  16. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...Yang J, Yang S (2015) Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl ...system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https...

  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

  18. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of ...A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody ...

  19. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011...al. "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation...
Showing: 1 - 20 of 57 results