... outperformed other 3D chromatin methods such as ChIA-PET or Hi-C, by increasing both the number of unique...

...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to ...

...devastating afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research Scientist ...mechanisms behind the disease are unclear. Dr. Di Schiavi plans on using C. elegans as a model to identify...disruption. As part of his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 ...behind SMN1 and its interaction partners, Dr. Di Schiavi will be able to better choose which genes and pathways...SMN1 and its partners in mouse cell lines. Dr. Di Schiavi hopes that these studies will accelerate the development...Website.  We are proud to provide support to Drs Di Schiavi and Nabavi and will continue to work to help those...

...Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying the abundance...time is time consuming and unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler's lab at...from each bin are then extracted and sequenced. Chiasson is currently using VAMP-seq to study vitamin K...scales and making personalized medicine a reality,” Chiasson explains. Elizabeth Unger: Developing a serotonin...

...organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. ...2017). Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli -...environments (Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID. E. coli is often a...

...create unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter...first described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification..., Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated...counter-selectable marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-...

...vulgaris, Erwinia carotovora, E. chrysanthemi, Escherichia coli, Gluconacetobacter xylinus, Gluconobacter...E. herbicola, E. rubrifaciens, E. stewartii, Escherichia coli, Klebsiella spp., Legionella pneumophila...spp., Brucella spp., Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus, Paracoccous ...integron promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens. 3 Biotech...

...instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186...

...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

...Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated...enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015)...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed...

... in: A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation... in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial UAG codon ... Described in: Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara...

...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...

...single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a) to begin to characterize ...et al., 2017). They characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion...

...Yang J, Yang S (2015) Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System. Appl ...system for CRISPR-Cas9-assisted genome editing in Escherichia coli. Acta Biochimica et Biophysica Sinica. https...

...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

...levels in general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

...anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of ...A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody ...

...-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011...al. "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation...

...PMCID: PMC307639. 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald...recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000...

...Mammalian/Lentiviral BbsI none S. pyogenes Puro Ochiai pSB700 64046 Mammalian/Lentiviral none S. pyogenes...simultaneously repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions for other...