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pRS_1N_400

Plasmid

#170476

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

Insert

T7A1, O1, O-, Lambda t1 in lacZ fragment

Expression

Bacterial

Promoter

T7A1

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Plasmid

#137110

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 AAAA

Plasmid

#87600

Purpose

Encodes Prp5 AAAA for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

D136A, P137A, L138A, D139A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 E235A

Plasmid

#87601

Purpose

Encodes Prp5 E235A for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

E235A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 N399D

Plasmid

#87602

Purpose

Encodes Prp5 N399D for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N399D

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 TAG

Plasmid

#87603

Purpose

Encodes Prp5 SAT motif to TAG for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

S446T, T448G

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP2

Plasmid

#87591

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp2

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP2 (PRP2 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335R

Plasmid

#87299

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335R

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335R

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 P369E

Plasmid

#87277

Purpose

Encodes Hsh155 P369E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

P369E

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K335E

Plasmid

#87273

Purpose

Encodes Hsh155 K335E for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335E

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L

Plasmid

#87269

Purpose

Encodes Hsh155 R294L for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294C

Plasmid

#87268

Purpose

Encodes Hsh155 R294C for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 Y292C

Plasmid

#87267

Purpose

Encodes Hsh155 Y292C for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-YSF3

Plasmid

#87598

Purpose

Encodes Y2H Gal4 DNA binding domain for Ysf3

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

YSF3 (YSF3 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-BUD13

Plasmid

#87547

Purpose

Encodes Y2H Gal4 DNA binding domain for Bud13

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

BUD13 (BUD13 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-SLU7

Plasmid

#87597

Purpose

Encodes Y2H Gal4 DNA binding domain for Slu7

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

SLU7 (SLU7 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP28

Plasmid

#87595

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp28

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP28 (PRP28 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP11

Plasmid

#87594

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp11

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP11 (PRP11 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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