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BCTVΔIR-pUPD2

Plasmid

#240717

Purpose

BCTV complementary sense ORFs lacking the IR. Has C1 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV CS

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVS-pUPD2

Plasmid

#240718

Purpose

BCTV IR lacking virion sense ORFs.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

BCTV IR

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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RT B2-B6-pUPD2

Plasmid

#240719

Purpose

GUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B2-B6 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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RT B6-pUPD2

Plasmid

#240720

Purpose

GUS:NPTII repair template used for HDR experiments with compatible reporter lines. Has B6 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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ZFN-pUPD2

Plasmid

#240721

Purpose

ZFN that targets the broken GUS:NPTII reporter with B2-B5 Goldenbraid grammar.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

ZFN

Use

Synthetic Biology

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAG304-ccdbNLUC

Plasmid

#249736

Purpose

Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pACYC-PJ23100-dpGFP

Plasmid

#249257

Purpose

An avGFP mutant designed by DeepDE that achieved a remarkable 74.3-fold increase in activity over four rounds of evolution, far surpassing the benchmark superfolder GFP.

Depositor

Xiaofeng Yang

Article

Li et al iScience. 2025 Aug 7;28(9):113324. doi:

Insert

dpGFP

Expression

Bacterial

Promoter

PJ23100

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1370_pTet-ECOR31_Hachiman

Plasmid

#223364

Purpose

Expression of ECOR31 Hachiman under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1465_pTet-ECOR31Ham_DELhamB_Mut

Plasmid

#223366

Purpose

Expression of ECOR31 Hachiman HamA (∆HamB) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamA (∆HamB)

Expression

Bacterial

Mutation

∆HamB

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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