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pSU19mCherry

Plasmid

#225181

Purpose

Medium copy cloning vector with mCherry integrated in the multiple cloning site. There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM255

Plasmid

#226899

Purpose

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

mEOS3.2-GS-MBP-10xN-Pex15(1-309)-Prescission-GSS-…

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM249

Plasmid

#226893

Purpose

Pex15(43-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Tags

prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 43-309)

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1-Myc-MBP-HA

Plasmid

#222709

Purpose

Expression AtTIR1-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1 (TIR1 Mustard Weed)

Tags

myc

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-AtIAA6-HA

Plasmid

#222710

Purpose

Expression MBP-AtIAA6-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtIAA6 (IAA6 Mustard Weed)

Tags

HA and MBP

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVN

Plasmid

#223861

Purpose

4G-cloning donor for mammalian promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-GST-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-MBP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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